骨保护素对骨-腱愈合促进作用的研究

Augmentation of tendon-bone healing with osteoprotegerin

目的:探讨骨保护素(OPG)在膝关节前交叉韧带重建术后骨腱愈合过程中的作用。方法:将24只成年新西兰白兔随机分成2组,OPG组与空白对照组。制备双膝关节前交叉韧带模型,分别于术后3周、6周和12周末每组各处死4只实验动物,取得完整膝关节标本,2只用于光镜下观察骨-腱愈合情况;2只用于行生物力学检测。结果:空白对照组与骨保护素组比较,组织学结果显示:在术后相同时间,OPG组骨-腱结合面有更多的成骨细胞生成。生物力学实验结果:在术后6周和12周末的生物力学试验结果均存在统计学差异(均P<0.01)。结论:骨保护素对膝关节前交叉韧带重建术后骨-腱愈合具有显著的促进作用。

Objective：To investigate the role of osteoprotegerin（OPG）in the knee after cruciate ligament reconstruction tendon bone healing process in the facilitating.Methods：The 24 adult New Zealand white rabbits were randomly divided into two groups （Blank control group and OPG group）,and we executed 4 rabbits,respectively in 3weeks、6 weeks and 12 weeks,to get the complete kee joint specimens.2 specimens were used to check for the histologic change and 2 specimens for biomechanical testing.Results：Histological results：at the same time,there were more osteoblast in OPG group.Six weeks and 12 weeks after operation specimens showed that two groups have significant differences.Conclusion：OPG can betterly promote bone-tendon healing after anterior cruciate ligament reconstruction.