Visfatin对多囊卵巢综合征大鼠肝脏胆固醇代谢的影响

Effect of Visfatin on Hepatic Cholesterol Metabolism in Rats with Polycystic Ovarian Syndrome

目的探讨visfatin对多囊卵巢综合征(PCOS)大鼠肝脏胆固醇合成的影响及机制。方法应用DHEA皮下注射制备PCOS模型。30只雌性SD大鼠随机分成正常对照组(NC组)10只、PCOS模型组20只,其中10只单次注射visfatin干预(PCOS+visfatin组)。结束实验后RT-PCR检测肝脏HMGCOA还原酶和SREBP-2的mRNA表达变化。结果 PCOS组大鼠肝脏组织HMG COA还原酶和SREBP-2的RNA表达水平显著高于正常对照组。给予visfatin干预后PCOS大鼠肝脏组织HMG COA还原酶和SREBP-2的mRNA表达水平显著高于PCOS大鼠肝脏组织的表达。结论 Visfatin可能通过促进SREBP-2和HMGCOA还原酶的表达来参与肝脏的胆固醇代谢。

Objective To study the effect of visfatin on hepatic cholesterol synthesis and mechanism in rats with polycystic ovary syndrome （PCOS）. Methods 30 female SD rats were randomly divided into normal control group （NC group = 10） 10 and PCOS group （n = 20）. PCOS model was induced by subcutaneous injecting dehydroeplandrosterone （DHEA） up to 20 days. Then single injection of visfatin intervention was given in 10 PCOS rats （PCOS ＋ visfatin group）. Hepatic HMG-CoA reductase and sterol regulatory element binding protein 2（SREBP-2） mRNA were evaluated by RT-PCR. Results HMG-CoA reductase mRNA level and SREBP-2 level in the PCOS group was significantly higher than that in the normal control group, After the intervention of visfatin, the expression of HMG-CoA reductase mRNA level and SREBP-2 in liver tissue of PCOS group increased. Conclusions Visfatin may involve in hepatic cholesterol metabolism by promoting the expression ofHMGCOA reductase and SREBP-2.