基于点击反应的纳米金比色检测还原糖的研究

Colorimetric Detection of Reducing Sugar Based on Gold Nanoparticles via Click-reaction

以纳米金为显色信号元件,利用点击反应建立一种简单、快捷、灵敏度高的还原糖检测方法.首先,利用巯基丙酸修饰纳米金,进而通过酰胺反应分别在纳米金上固定炔基和叠氮基,构建两种不同的功能性纳米金,从而实现对还原糖的定量检测.考察了温度、时间等因素对点击反应效率的影响,结果表明,在70℃条件下反应10 min,还原糖的浓度与吸光度比值A650/520之间呈现良好的线性关系,检出限达到0.0015 μmol/L.本方法可直接用于蜂蜜样品的检测,加标回收率为98.1％～103.6％.

A simple, rapid, and high-sensitive method for the detection of reducing sugar was established based on the click-reaction with gold nanoparticles （AuNP） as the chromogenic signal component. The quantitative detection of reducing sugar was accomplished by building two different kinds of functionalized AuNPs. The two AuNPs were got from a two-step reaction which were first modified with mercaptopropionic acid, and then fixed with alkynyl or azide group via amidation reaction, respectively. Meanwhile, the effects of reaction temperature and time on the efficiency of click-reaction have been examined. The results showed good linear correlation between the absorbance ratio A650/520 and the concentration of reducing sugar reacted at 70 ℃ for 10 min, with a detection limit of the method was 0. 0015 μmol/L. This method can be directly applied for the analysis of honey samples with the recoveries of 98.1%-103.6%.