超高效液相色谱法测定浓缩当归丸和当归药材中光毒性化合物香豆素

Determination of Light Toxic Compound Coumarin in Enrichment Angelica Pills and Angelica Sinensis by Ultra Performance Liquid Chromatography

建立了用超高效相液相色谱法同时测定浓缩当归丸和当归药材中光毒性化合物补骨脂素、花椒毒素和佛手柑内酯含量的方法.样品用甲醇超声提取后,用Waters ACQUITY BEH C18柱（100 mm×2.10 mm,1.7 μm）分离,以乙腈-水做流动相,梯度洗脱,柱温为30℃,流速为0.45 mL/min,检测波长为245 nm,进样量为3μL.结果表明,在一定的浓度范围内,补骨脂素、花椒毒素和佛手柑内酯的浓度与峰面积线性关系良好,相关系数r为0.9992 ～0.9996,样品的加样回收率为96.20％ ～102.42％,RSD为1.5％～3.0％.本方法简便、快速,精密度高、重现性好.本方法可用于浓缩当归丸和当归药材中光毒性化合物补骨脂素、花椒毒素和佛手柑内酯的含量测定.

Abstract An ultra performance liquid chromatography （UPLC） method was developed for the simultaneous determination of psoralen, xanthotoxin and bergapten in Enrichment angelica pills and Angelica sinensis. The samples were extracted with methanol. The target compounds were seperated on Waters ACQUITY BEH C18 （100 mm × 2.10 mm,1.7 μm） , with a gradient acetonitrile-water solvent system at 30 ℃, the flow rate was 0.45 mL/min, the detection wavelength was set at 245 nm and the injection volume was 3 μL. Results showed the good linear ranges for psoralen, xanthotoxin and bergapten were within 2.34-67.4 mg/L, with correlation coefficients（r） of 0. 9992-0. 9996, the mean recoveries were in the range of 96.20%-102.42% , with the relative standard deviation of 1.5%-3.0%. The method is simple, rapid, accurate and reliable, and suitable for the determination of psoralen, xanthotoxin and bergapten.