葡萄籽提取物原花青素抗氧化作用量效关系研究

Antioxidative Dosage-effect Relationship of Grape Seed Proanthocyanidin Extract

目的观察葡萄籽提取物原花青素抗氧化作用的量效关系。方法实验小鼠随机分为空白组、模型组和原花青素5 mg/kg组、15 mg/kg组、45 mg/kg组、135 mg/kg组、405 mg/kg组。模型组和各给药组小鼠腹腔注射D-半乳糖120 mg/kg制备氧化损伤模型,空白组注射等体积生理盐水溶液,造模期间各组动物给予相应药物,连续造模7周。给药结束后,测定血清中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性,观察受试物的量效关系。结果原花青素45、135、405 mg/kg剂量组可显著降低模型小鼠血清MDA水平,原花青素15、45、135、405 mg/kg剂量组血清SOD活性提高。结论原花青素在>15 mg/kg剂量时对小鼠D-半乳糖氧化损伤模型显示出较好的抗氧化活性,提示临床应保证一定的服用剂量以发挥较好的抗氧化功效。

Objective To observe the antioxidative dosage-effect relationship of grape seed proanthocyanidin extract （GSPE）. Methods The mice were randomly divided into seven groups： blank group, model group, 5 mg/kg GSPE group, 15 mg/kg GSPE group, 45 mg/kg GSPE group, 135 mg/kg GSPE group and 405 mg/kg GSPE group. The mice in blank group were dealt with saline solution by intraperitoneal injection, the others were dealt with D-galactose （120 mg/kg） by intraperitoneal injection for seven weeks to make oxidative damage model. Meanwhile, the mice were given corresponding dose of the drug. Subsequently the level of malondialdehyde （MDA） and activity of superoxide dismutase （SOD） in the serum were measured to observe the antioxidative dosage-effect relationship of GSPE. Results The 45, 135, 405 mg/kg GSPE group reduced the MDA level, and the 15, 45, 135, 405 mg/kg GSPE group increased the SOD activity. Conclusion GSPE has significant antioxidant activity on mice dealt with D-galactose above the dose of 15 mg/kg, suggesting that the clinical use of GSPE should guarantee a certain dose to play a good antioxidant effect.