RP-HPLC同时测定广金钱草中的vicenin-1、夏佛塔苷和vicenin-3

Determination of vicenin-1,schaftoside and vicenin-3 in Desmodium styracifolium by RP-HPLC

目的采用反相HPLC法同时测定广金钱草中vicenin-1、夏佛塔苷和vicenin-3的含量。方法采用Kromasil RPC18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,流动相A相为水-0.1%甲酸水溶液、B相为甲醇,梯度洗脱:0~8 min、30%~32%B,8~32 min、32%~34%B,32~60 min、34%~40%B,流速1.0 mL·min-1,检测波长272 nm。结果 vicenin-1在0.230~1.380μg(r=0.9998)、夏佛塔苷在0.702~1.872μg(r=0.9998)、vicenin-3在0.220~1.320μg(r=0.9999)的线性关系良好;平均回收率(n=6)分别为99.44%、99.58%、100.01%。结论所用方法具有简便、快速准确、重复性好的特点,可为广金钱草药材的质量控制提供依据。

OBJECTIVE To establish an RP - HPLC method for simultaneous determination of vicenin - 1, schafloside and vicenin -3 in Desmoclium styracifolium. METHODS The determination was carried out on a Kromasil RP - C lS column （250 mm x 4. 6 mm,5 p^m ） with the column temperature set at 30 ~C. The mobile phase consisted of 0.1% aqueous formic acid （A） and metha- nol（B）. A gradient program was used according to the following profile：0 -8 min,30% -32% B;8 -32 rain,32% -34% B;32 -60 min,34% -40% B. The detection was carried out at 272 nm,and the flow rate was kept at 1.0 mL-min-l. RESULTS The method showed a good linear relationship within the range of 0.230 - 1. 380 p^g for vicenin - 1 （ r = 0. 9998 ）, 0. 702 - 1. 872 ixg for schaftoside （ r =0.9998 ） and 0.220 - 1. 320 g for vicenin - 3 （ r = 0. 9999 ） ; the average recovery was 99.44% for vicenin - 1, 99.58% for schaftoside and 100.01% for vicenin - 3. CONCLUSION The method is simple, rapid and accurate with good reproducibility, which provides the basis for quality control of Desmodium styracifolium.