摘要
目的采用超高效液相色谱法快速测定脉络通胶囊中葛根素的含量。方法采用Poroshell 120SB—C18(100mm×2.1mm,2.7μm)色谱柱,以甲醇一水为流动相进行梯度洗脱,流速0.4mL·min-1,检测波长250nm,柱温25℃,采集频率40Hz,进样体积1μL。结果葛根素0.09~0.45mg·mL-1与峰面积的线性关系良好(r=0.9999),平均回收率为100.34%(RSD=0.90%,n=6)。结论所用方法简便、准确、专属性强、重复性好,可用于脉络通胶囊中葛根素的含量测定。
OBJECTIVE To establish an ultra - performance liquid chromatography (UPLC) method for determination of puerarin in Mailuotong capsules. METHODS The separation was perfomied on a Poroshell 120 SB - C18 ( 100 mm x 2.1 mm,2.7 txm) column. The mobile phase was methanol - water with gradient elution at a flow rate of 0.4 mL. min - 1. The detection wavelength was 250 nm, and the column temperature was 25 ℃. The injection volume was 1 μL, and the collection frequency was 40 Hz. RESULTS The liner range was 0.09 - 0.45 mg mL- 1 ( r = 0.9999) , the recovery of method was 100.34% , and the RSD was 0.90% ( n = 6 ). CONCLU- SION The method is simple, accurate, and selective for the determination of puerarin content in Mailuotong capsules.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2013年第6期621-622,共2页
West China Journal of Pharmaceutical Sciences
关键词
超高效液相色谱法
脉络通胶囊
葛根素
含量测定
Ultra - performance liquid chromatography (UPLC)
Mailuotong capsules
Puerarin
Assay