正交实验优选赤小豆萌芽工艺研究

Study on Optimization Technology of Vigna umbellata Germination by Orthogonal Test

以9个产地的赤小豆为试材,以代表抗过敏活性的透明质酸酶抑制率为指标,在产地筛选的基础上,对光照、温度、水分3个因素通过L9(33)正交实验,以期优化赤小豆萌芽工艺条件。结果表明:在无光照、温度30℃、浇灌次数3次/d的萌芽条件下,赤小豆提取物透明质酸酶抑制率为(90.67±4.82)%。该试验工艺设计合理、结果可靠,为赤小豆萌芽抗敏功效研究提供了参考依据。

Taking 9 origin of Vigna umbellata as materials, hyaluronidase inhibition rate that represent anti-allergic effect, based on screening of origin, the factors of sunlight,temperature and moisture were investigated by I4 （3a） orthogonal test,in order to optimize the technology for Vigna umbellata sprouts. The results showed that, under the conditions for Vigna umbellata germination for at 30℃ with no illumination and irrigation 3 times/day,hyaluronidase inhibition rate reached （90. 67±4. 82）%. The experiment method was reasonable and stable,could be provide reference for anti-allergic activity extracted from Vigna umbellata sprouts.