“红颜”草莓茎尖组织培养快繁技术研究

Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe'

以"红颜"草莓苗为试材,以茎尖为外植体,以MS为基础培养基,研究了最佳激素配比及最佳移栽基质对"红颜"草莓苗生长的影响,以期建立一个高效的茎尖组织培养快繁技术体系。结果表明:适宜的初代培养基为MS+6-BA 0.5mg/L+NAA 0.1mg/L+蔗糖30g/L+琼脂5g/L,萌芽率为81%;适宜的增殖培养基为MS+6-BA 0.25mg/L+NAA 0.1mg/L+蔗糖30g/L+琼脂5g/L,增殖系数为9.1;最适宜的生根培养基为1/2MS+NAA 0.1mg/L+蔗糖30g/L+琼脂5g/L,生根率为98.0%;最佳移栽基质为营养土∶珍珠岩∶蛭石=1∶1∶1,移栽成活率达到97.7%。

Taking the Fragaria X ananassa DDuch ＇ Benihoppe＇ as the experimental material, the shoot tip as explants, MS as the basic medium, the optimal proportion of hormones and best transplanting substrate on growth of strawberry plantlets confidante were studied,in order to establish an efficient system of rapid propagation technique of cultivating a tip of stem tissue. The results showed that the suitable medium for primary MS＋6-BA 0. 5 rng/L＋NAA 0. 1 mg/L＋ sucrose 30 g/L＋agar 5 g/L,germination rate was 81% ;suitable proliferation culture medium for MS＋6-BA 0. 25 mg/L＋ NAA 0. 1 mg/L＋sucrose 30 g/L＋agar 5 g/L,mutiplication coefficent was 9.1. The most suitable rooting medium was 1/2 MS＋NAA 0. 1 mg/L＋sucrose 30 g/L＋agar 5 g/L,the rooting rate was 98. 00%. The best transplanting matrix ： nutrition soil ： perlite ： vermiculite= 1 ： 1 ： 1 ,transplanting survival rate reached 97.7%.