稀土离子介导下呋喃香豆素类药物与溶菌酶的作用机理

Interaction of Furanocoumarin Drugs and Lysozyme in the Presence of Rare Earth Ions

在模拟人体生理条件下,采用荧光光谱法和同步荧光光谱法研究稀土离子La3+、Gd3+、Yb3+和Lu3+介导下,补骨脂素、异补骨脂素、5-甲氧基补骨脂素和8-甲基补骨脂素四种呋喃香豆素类药物与溶菌酶(LYSO)的作用机理。荧光光谱结果表明,稀土离子的存在使得四种呋喃香豆素类药物对LYSO的荧光猝灭增强,其猝灭机理主要为静态猝灭。四种呋喃香豆素类药物与LYSO均形成1∶1复合物,其作用力主要为氢键和范德华力。不同的稀土离子对四种呋喃香豆素类药物与LYSO相互作用的介导能力不同,其顺序为:La3+>Gd3+>Yb3+>Lu3+。同步荧光光谱结果显示稀土离子介导下,四种呋喃香豆素类药物与LYSO相互作用后,LYSO的构象均发生改变。

Under simulated human physiological conditions, the mechanism of interaction between four furanocoumarin drugs and lysozyme（LY80） was investigated using the fluorescence spectroscopy and synchronous fluorescence spectroscopy in the presence of rare earth ions（La3＋ , Gd3＋ , yb3＋ and Lu3＋ ）. The results of fluorescence spectroscopy revealed that four furanocoumarin drugs enhanced the fluorescence quenching of LYSO in the presence of rare earth ions. The mechanism of fluorescence quenching was static quenching. The 1 ＇ 1 complex was formed between each furanocoumarin drug and LYSO. The hydrogen bond and Vander Waals force were the major driving force between four furanocoumarin drugs and LYSO. Different rare earth ions had different ability to mediate the interaction of four furanocoumarin drugs and LYSO. The order was as follows ： La3＋ 〉 Gda＋ 〉 yh3＋ 〉 Lu3＋. The results of synchronous fluorescence spectroscopy indicated that the conformation of LYSO was changed by four furanocoumarin drugs in the presence of rare earth ions.