高效液相色谱法同时测定不同产地及不同药用部位景天三七中4种黄酮类成分的含量

Simultaneous Determination of Four Flavonoids in Sedum aizoon L.from Different Origin and Medicinal Parts by High Performance Liquid Chromatography

建立了同时测定不同产地及不同药用部位景天三七中槲皮素、木犀草素、山奈酚和异鼠李素含量的高效液相色谱(HPLC)法。采用TOP ODS-AQ色谱柱(250×4.6mm,5μm),以甲醇-0.1%磷酸溶液为流动相梯度洗脱,检测波长为365nm。结果表明,槲皮素、木犀草素、山奈酚和异鼠李素的浓度分别在1.90~189.90μg·mL-1(r=0.99996),1.12~112.00μg·mL-1(r=0.99998),3.71~370.56μg·mL-1(r=0.99995)和0.98~97.60μg·mL-1(r=0.99996)范围内与其色谱峰面积呈良好线性关系;平均加标回收率分别为99.79%、100.06%、100.19%和100.00%,且不同产地及不同药用部位的4个黄酮类成分在数量上或质量上有明显差异。该方法快速、准确,重现性好,可用于同时测定景天三七中槲皮素、木犀草素、山奈酚和异鼠李素含量。

A high performance liquid chromatography method for simultaneous determination of quercetin,luteolin,kaempferol and isorhamnetin in Sedum aizoon L. from different origin and medicinal parts was established. A TOP ODS-AQ column（250）〈4.6 ram,5 t^m） was adopted using methanol（A）- 0. 1% phosphoric acid solution（B） as the mobile phase with gradient elution. Quercetin, luteolin, kaempferol and isorhamnetin showed good linearity in the ranges of 1.90-189.90 μg mL-1 （r = 0. 99996）,1.12-112.00 μg.mL-1 （r=0. 99998） ,3.71-370.56 μg.mL-1 （r=O. 99995）and 0.98-97.60 μg mL-2 （r=0. 99996） ,respectively. The average recoveries （n----6） varied from 99.79% to 100.19%. The four flavonoids of Sedum aizoon L. from different origin and medicinal parts showed obvious differences in the quantity or quality. This method is rapid, accurate with good reproducibility, which can be used for the determination of quercetin,luteolingkaempferol and isorhamnetin in Sedum aizoon L.