纳米铂与牛血清白蛋白相互作用的光谱研究

Investigation of Interaction between Platinum Nanoparticles and Bovine Serum Albumin by Spectroscopy

应用荧光光谱法和紫外-可见吸收光谱法研究了纳米铂(PtNP)与牛血清白蛋白(BSA)之间的相互作用。研究发现,PtNP与BSA猝灭机制为静态猝灭。求得了不同温度下PtNP与BSA之间相互作用的结合常数K≈104,结合位点数n≈1,根据热力学参数值确定了它们之间的作用力为疏水作用力。同时应用同步荧光光谱考察了PtNP对BSA构象的影响。

The interaction between platinum manoparticles（PtNP） and bovine serum albumin（BSA） was studied by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results showed that PtNP caused the fluorescence quenching of BSA through a static quenching procedure. The binding constant（K~ 104） and binding site （n=1） between PtNP and BSA were obtained. According to the values of thermodynamic functions, the type of force between PtNP and BSA was hydrophobic interaction. At the same time,the effect of PtNP on the conformation of BSA was investigated by using synchronous fluorescence spectroscopy.