超高效液相色谱-串联质谱快速测定卷烟主流烟气中特有亚硝胺

Rapid Determination of Tobacco-Specific Nitrosamines in Mainstream Cigarette Smoke Use Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了超高效液相色谱一串联质谱（UPLC—MS／MS）测定烟草中特有亚硝胺（TSNAs）的分析方法。用剑桥滤片捕集卷烟主流烟气总粒相物，乙酸铵提取总粒相物，在C18柱上以甲醇-0．1％乙酸溶液和0．1％乙酸水溶液为流动相进行梯度洗脱，流速0．4mL／min，4种烟草特有亚硝胺化合物在1min内得到良好分离，采用电喷雾正离子多反应监测模式（MRM）扫描定性，内标法定量。方法线性范围为0．5～200ng／mL，定量限（S／N-10）为0．009～0．053ng／cig。该方法用于检测卷烟中主流烟气中特有亚硝胺，操作简单快捷，重现性好，回收率在91．1％～106．2％之间。

A new method has been developed for the determination of tobacco-specific nitrosamines （TSNAs） in mainstream cigarette smoke using ultra performance liquid chromatography-tandem mass spectrometry. Total particulate matters in mainstream smoke were trapped by Cambridge filter pad and extracted by ammonium acetate. On a C18 column, four tobacco-specific nitrosamines was separated within 1 min with gradient elution using water and methanol containing 0.1~ acetic acid as the mobile phase at a flow rate of 0. 4 mL/min. Analyzed by positive electrospray ionization （ESI＋）, the linear ranges were 0.5-200 ng/mL and the quantitation limits were 0. 009-0. 053 ng/cig（S/N=10）. The proposed method has been applied to the determination of TSNAs in mainstream cigarette smoke in cigarettes with recoveries of 91.1%-106.2%.