摘要
目的:建立测定颈复康颗粒中盐酸小檗碱含量的方法。方法:采用高效液相色谱法。色谱柱为Kromasil C18柱,流动相为乙腈-0.05mol/L磷酸二氢钾溶液(用磷酸调节pH至3.0,30:70,V/V),检测波长为350nm,流速为1.0ml/min,柱温为30℃,进样量为20μl。结果:盐酸小檗碱进样量在0.0244~0.488gg范围内与峰面积积分值呈良好的线性关系(r=0.9999);精密度、稳定性、重复性试验的RSD≤1.33%;平均加样回收率为99.0%,RSD=0.57%(n=6)。结论:该方法简单、准确、重复性好,可用于颈复康颗粒的质量控制。
OBJECTIVE: To establish a method for the content determination of berberine hydrochloride in Jingfukang granules. METHODS : HPLC method was adopted. The determination was performed on Kromasil C18 column with mobile phase consisted of acetonitrile-0.05 mol/L sodium hydrogen phosphate (pH value adjusted to 3.0 using phosphoric acid, 30 : 70, V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 350 nm, and the column temperature was 30 ℃. The injection volume was 20 μl. RESULTS: The linear range of berberine hydrochloride was 0.024 4-0.488 μg (r=0.999 9) with an average recovery of 99.0% (RSD=0.57%, n=6). RSDs of precision, stability and reproducibility tests were lower than 1.33%. CONCLUSIONS: The method is simple, accurate and reproducible and can be used for the quality control of Jingfukang granules.
出处
《中国药房》
CAS
CSCD
2013年第48期4586-4588,共3页
China Pharmacy