GC法同时测定跌打万花油中4种成分的含量

Simultaneous Determination of the Content of 4 Components in Dieda Wanhua You by GC

目的：建立同时测定跌打万花油中樟脑、薄荷脑、冰片和水杨酸甲酯含量的方法。方法：采用气相色谱法，以挥发油测定器蒸馏制备供试品溶液。色谱柱为安捷伦DB-WAX极性毛细管色谱柱，采用程序升温，检测器为氢火焰离子化检测器，进样口温度为210℃，检测器温度250℃，栽气为氮气，流速为73．5ml／min，不分流进样，进样量为1μl。结果：樟脑、薄荷脑、冰片（异龙脑和龙脑）、水杨酸甲酯的检测质量浓度分别在0．0623～1．993、0．25～8．011、0.444～14．221、0．373-11．956mg／ml范围内与峰面积积分值呈良好的线性关系（r均为0．9996）；精密度、稳定性、重复性试验的RSD≤3．22％；平均加样回收率分别为101．11％、103．14％、100．60％、102．04％，RSD分别为2．19％、2．03％、2．15％、2．88％（n=6）。结论：该方法重复性好、灵敏度高、结果准确，可用于跌打万花油的质量控制。

OBJECTIVE： To establish a method for the content determination of camphor, menthol, synthetic borneol and methyl salicylate in Dieda wanhua you. METHODS： GC was applied to quantitative analysis. Test solution was prepared by distillation with volatile oil detector. Using temperature programmed, FID as detector and nitrogen as carrier gas, the experimented column was Agilent DB-WAX polar capillary column with splitless injection at the flow rate of 73.5 ml/min. The injector temperature was 210℃ and detector temperature was 250 ℃. The injection volume was 1 μl. RESULTS： The linear ranges of camphor, menthol, synthetic bomeol （isoborneol and borneol） and methyl salicylate were 0.062 3-1.993, 0.25-8.011, 0.444-14.221 and 0.373- 11.956 mg/ml respectively （r=0.999 6）. RSDs of precision, stability and reproducibility tests were all lower than 3.22%. The average recoveries were 101.11% （RSD=2.19%, n=6）, 103.14% （RSD=2.03%, n=6）, 100.60% （RSD=2.15%, n=6） and 102.04% （RSD=2.88%, n=6）, respectively. CONCLUSIONS： The method is reproducible, sensitive, accurate and suitable for the quality control of Dieda wanhua you.