中空纤维离心超滤-HPLC法测定茵栀黄口服液中绿原酸的含量

Content Determination of Chlorogenic Acid in Yinzhihuang Oral Liquid by Hollow Fiber Based Centrifuge Ultrafitration-HPLC

目的：建立测定茵栀黄口服液中绿原酸含量的方法。方法：采用高效液相色谱法，样品经中空纤维离心超滤法处理后进行测定。色谱柱为Sapphire C18柱，流动相为乙腈-0．4％磷酸溶液（15：85，v／v），检测波长为327nm，流速为1．0ml／min，进样量为20μl，柱温为25℃。结果：绿原酸检测质量浓度在7．5-120μg／ml范围内与峰面积积分值呈良好的线性关系（r=0.9999）；精密度、稳定性、重复性试验的RSD≤1．6％；平均加样回收率为98．5％，RSD=1．5％（n=6）。结论：该方法操作简便、结果准确、经济、环保，有利于更加客观、全面地评价及控制茵栀黄口服液的质量。

OBJECTIVE： To establish a method for the content determination of chlorogenic acid in Yinzhihuang oral liquid. METHODS： A hollow fiber based centrifuge ultrafitration pretreatment procedure combined with HPLC was applied to purify solution samples. The separation was carried out on a Sapphire C18 column with acetonitrile-0.4% phosphoric acid solution （15 ： 85, V/V） as mobile phase at flow rate of 1.0 ml/min. The detection wavelength was set at 327 nm, the sample size was 20 μl, and the column temperature was set at 25 ℃. RESULTS： The linear range for chlorogenic acid was 7.5-120 μg/mL （r=0.999 9） with an average recovery of 98.5% （RSD=-1.5%, n=6）. RSDs of precision, stability and reproducibility tests were all lower than 1.6%. CONCLUSIONS： The method is simple, accurate, economical and environmental, and contributes to objective and comprehensive quality evaluation and control of Yinzhihuang oral liquid.