量子点对泌尿系肿瘤活细胞的毒性效应

The cytotoxic effect of quantum dots on the urinary tract tumor live cells

目的 探讨水溶性量子点硒化镉/硫化锌（CdSe/ZnS）对泌尿系肿瘤活细胞的毒性效应,观察其对泌尿系肿瘤细胞生长的影响.方法 将不同浓度的量子点（5、10、20、40、80 nmol/L）分别作用于膀胱癌EJ细胞和前列腺癌PC-3细胞,通过细胞计数试剂盒（CCK-8）检测法来评价其对肿瘤细胞活性的影响.结果 CCK-8检测发现,两种活体细胞和量子点共同孵育24 h后,其存活率随着量子点浓度的升高均显示下降趋势,在量子点通常使用浓度（5、10、20 nmol/L）下,两种细胞的存活率分别为101.0％、99.2％、97.9％（EJ细胞）和100.1％、101.0％、99.0％（PC-3细胞）,与对照组比较差异无统计学意义（P＞0.05）,而在80 nmol/L高浓度时,两者存活率和对照组比较差异有统计学意义（P＜0.001）,但是两种细胞的平均存活率也高达92.6％（EJ细胞）和95.8％（PC-3细胞）.结论 高浓度的量子点对膀胱癌EJ和前列腺癌PC-3细胞的活性有一定程度的影响,但是通常使用浓度的量子点对它们的存活率几乎没有影响.

Objective To detect the cytotoxic effect of quantum dots（CdSe/ZnS QDs） on the urinary tract tumor cells,Study the influence of CdSe/ZnS quantum dots on growth of the urinary tract tumor cells.Methods The cytotoxic effect of QDs in different concerntrations （5,10,20,40,80 nmol/L） was detected on bladder cancer cell line EJ and prostate cancer cell line PC-3 by cell counting kit-8 （CCK-8）,assessing the activities of tumor cells.Results Two kinds of live cells incubated with QDs for 24 hours,using CCK-8 test,performed that the survival rate of cells was decreased with the increasing of QDs concentrations.However,in the common concentration （5,10 and 20 nmol/L）,the survival rate of the two kinds of cells was 101％,99.2％,97.9％ （EJ cell line）and 100.1％,101.0％,99.0％ （PC-3 cell line） respectively.Moreover,no statistical difference in survival rate was observed between experimental and control groups （P ＞ 0.05） ; in the high concentration （80 nmol/L）,there were obvious difference between experimental and control groups （P ＜ 0.01）,however,the survival rates of bladder cancer cell line EJ and prostate cancer cell line PC-3 could still remained 92.6％ and 95.8％,respectively.Conclusion The quantum dots in high concentrations have a certain degree of influence on the activity of bladder cancer cell line EJ and prostate cancer cell line PC-3,however,for the conventional use,the concentration of quantum dots have little impact on their survival.