膀胱尿路上皮癌长链非编码RNA差异表达及意义

Study on long noncoding RNA in urothelial carcinoma of bladder

目的 探讨长链非编码RNA（LncRNA）在人类膀胱尿路上皮癌组织中的表达差异及意义.方法 收集手术切除12例尿路上皮癌及3例正常人膀胱尿路上皮黏膜,提取RNA,逆转录成cDNA并标记,在标准条件下将标记好的与芯片进行杂交,使用荧光扫描仪扫描荧光强度并分析基因表达谱差异.结果 采用Enhancer LncRNA profiling和Rinn LncRNAs profiling两种分析方法,分别发现了7条和25条差异表达的基因,并通过对其中3条差异表达基因进行逆转录-聚合酶链反应（RT-PCR）检测证实其表达与芯片结果一致.结论 LncRNA与膀胱尿路上皮癌发生、发展有着密切联系.

Objective To compare the gene expression differences between human urothelial carcinoma and normal bladder tissues,and the significances in utothelial carcinoma of bladder.Methods We collected 12 specimens of urothelial carcinoma and 3 specimens of normal bladder mucosa,then we extracted RNA,transcribe it into cDNA and labeled it.Then we used the probe which have already be labeled hybridize with microarray.After washing,slides were scanned with the Agilent DNA Microarray Scanner.Data was extracted using Agilent Feature Extraction software.Results Compared with nomarl bladder tissues,we found 7 differentially expressed genes by Enhancer long noncoding RNA （LncRNA） profiling and 25 differentially expressed genes by Rinn LncRNAs profiling.Then we used reverse transcriptase-polymerase chain reaction （RT-PCR） assay to amplify three of the differentially expressed genes,its expression consistent with the microarray results.Conclusion LncRNA have close relationship with urothelial carcinoma＇ s developing.It may be an significant tumor marker for urothelial carcinoma of bladder,which can help us make early diagnosis,prediction and open new paths of gene therapy for bladder urothelial carcinoma.