胃癌患者血清基质金属蛋白酶-9水平的变化

Serum matrix metalloproteinase-9 and its changes in patients with gastric carcinoma

目的 观察胃癌患者血清基质金属蛋白酶-9 （MMP-9）的表达,探讨其临床价值.方法 应用酶联免疫吸附试验（ELISA）法测定60例胃癌患者、30例正常人血清MMP-9水平,对36例胃癌患者手术前后血清MMP-9水平进行比较.结果 胃癌患者血清MMP-9水平[（60.44±9.75） μg/L]明显高于正常对照组[（26.79±2.65） μg/L],两者比较差异有统计学意义（P＜0.01）;血清MMP-9水平与胃癌患者性别、年龄和癌细胞分化程度无相关;血清MMP-9水平与胃癌患者临床分期有关,Ⅳ期[（72.47±4.21） μg/L]明显高于Ⅲ期[（65.73±4.36） μg/L]、Ⅲ期[（65.73±4.36）μg/L]明显高于Ⅱ期[（54.89±3.73）μg/L]、Ⅱ期[（54.89±3.73）μg/L]明显高于Ⅰ期[（48.42±3.89） μg/L]、两两比较差异有统计学意义（P＜0.05）;手术后胃癌患者血清MMP-9水平[（6.89±1.27） μg/L]明显低于患者手术前[（7.19±1.26）μg/L],两者差异有统计学意义（P＜0.01）.结论 MMP-9可作为反映胃癌疾病进展的分子标志物之一.

Objective To investigate the level of serum matrix metalloproteinase-9 （MMP-9） in patients with gastric carcinoma and study its clinical value.Methods Serum MMP-9 were detected with Enzyme linked immunosorbent assay （ELISA） in 60 cases of patients with gastric carcinoma and 30 cases of normal control subject,compared with 36 cases of patients with gastric carcinoma pre-and post-operation.Results The level of serum MMP-9 elevated in all subgroups of patients with gastric carcinoma （60.44 ± 9.75） μg/L was higher than that in the controls （26.79 ± 2.65） μg/L （P ＜ 0.01）,serum MMP-9 was not correlated with sex,age and histological grade; serum MMP-9 was correlated with TNM stage,the serum MMP-9 level in stage Ⅳ （72.47 ±4.21） μg/L was higher in comparison with stage Ⅲ （65.73 ±4.36） μg/L （P ＜0.01）,in stage Ⅲ （65.73 ±4.36） μg/L it was I higher than that in stage Ⅱ （54.89 ± 3.73） μg/L （P ＜ 0.01）,in stage Ⅱ （54.89 ± 3.73） μg/L it was higher than that in stage Ⅰ （48.42 ± 3.89） μg/L （P ＜ 0.05）,the serum MMP-9 levels were remarkably decreased in patients with gastric carcinoma after operation （P ＜ 0.01）.Conclusion MMP-9 may be used as a molecular marker to assess of the disease status of gastric carcinoma.