Sonic Hedgehog在非小细胞肺癌中的表达及其与患者生存周期的关系

Expression of Sonic Hedgehog in non-small cell lung cancer and its relationship with the cycle of life

目的 观察非小细胞肺癌患者Sonic Hedgehog（Shh）信号通路相关蛋白的表达,探讨在肺癌发展过程中的作用.方法 选择本院2013年1月至6月非小细胞肺癌手术患者46例,取切除肺癌组织为观察组,取癌旁组织39例,以非非小细胞肺癌患者28例肺部组织为对照组.采用免疫组织化学方法观察Shh和Gli2蛋白表达.结果 观察组Shh和Gli1蛋白阳性率分别为73.91％（34/46）和67.40％（31/46）,均高于癌旁组织和对照组（P＜0.05）,Ⅳ、Ⅲ期患者Shh和Gli1蛋白阳性率分别为80.95％（17/21）、71.43％ （15/21）和91.67％（11/12）、83.33％（10/12）,均高于Ⅰ期和Ⅱ期患者（P＜0.05）.结论 Shh信号通路可能通过激活Shh蛋白和Gli蛋白表达促进非小细胞肺癌组织异常增殖及细胞癌变.

Objective To observe the expression of Sonic Hedgehog （Shh） signaling pathway related protein in patients with non-small cell lung cancer and explore their roles in the development of lung cancer.Methods Forty-six patients with non-small cell lung cancer undergoing surgical operation were selected in our hospital from January to June,2013,which were operation resection of lung cancer as the observation group,39 cases the tissue of adjacent to carcinoma,28 cases of lung tissue with no lung cancer as control group.Using immunohistochemical method to observe the Shh and Gli2 protein expression.Results Shh and Gli1 protein in the cells were expressed in the cytoplasm under light microscope,yellow or brown yellow.The positive rate of Shh and Gli1 protein in the observe group were respectively 73.91％ （34/46） and 67.40％ （31/46）,were higher than that in paracancerous tissues and the control group （P ＜0.05）,The positive rate of Shh and Gli1 protein in Ⅲ,ⅣWstage were 80.95％ （17/21）,71.43％（15/21） and 91.67％ （11/12）,83.33％ （10/12）,were higher than those of Ⅰ and Ⅱ phase （P ＜ 0.05）.Conclusion In Shh signal pathway,there may be through the activation of Shh protein and the expression of Gli protein which promote carcinogenesis and cell proliferation in non-small cell lung cancer.