大鼠颞骨内段面神经缺血损伤模型的建立

A facial palsy model induced by ischemia of the intratemporal facial nerve

目的 通过损伤岩动脉建立建立大鼠面神经缺血模型,并根据术后行为学和面神经神经元存活数目以及轴突的变化探讨模型的可行性及稳定性.方法 60只SD大鼠,手术组40只行岩动脉横断术,对比正常组10只及假手术组10只,观察大鼠行为学表现,并行Nissl染色观察面神经核团位置、形态和存活数目以及用电镜观察面神经轴突及髓鞘的变化.结果 术后手术组大鼠均出现面瘫表现;手术组存活面神经元数目对比正常组和假手术组明显减少（P＜0.05）;手术组的轴突空泡化及髓鞘破坏所占的比例较正常组和假手术组明显增多（P＜0.05）.结论 此模型对血管定位准确,面神经元数目缺失及轴突变化明显.

Objective To establish the facial palsy model induced by the interruption of the petrosal artery in rats,and evaluate the feasibility and stability of the model by regarding their behavioral facial nerve function and the number of survival facial neurons and the changes of the axons.Methods The petrosal artery in 40 SD male rats was transected （operation group）,and control group and sham group were created （n =10 each）.The behavioral function of the rats （n =20） was analyzed.The distribution and morphology of the facial neuron and the number of survival facial neurons were also observed by Toluidine blue stain,and so were the axons by using the electron microscope.Results The rats had facial paralysis after operation,and the number of survival facial neurons was significantly decreased （P ＜ 0.05） and the proportion of myelinoclasis and vacuole formation was significantly increased in the operation group as compared with those in the control group and the sham group （P ＜ 0.05）.Conclusion The petrosal artery can be precisely located in proportion of myelinoclasis and vacuole formation,so this animal model can be helpful for the research on the ischemia of the intratemporal facial nerve.