血管紧张素Ⅱ及其受体拮抗剂在系膜细胞培养中对结缔组织生长因子表达的影响

EFFECT OF ANGIOTENSIN Ⅱ AND ITS RECEPTOR ANTAGONIST ON EXPRESSION OF CONNECTIVE TISSUE GROWTH FACTOR IN MESANGIAL CELLS

目的 :研究血管紧张素Ⅱ (AngⅡ )及其受体拮抗剂Losartan对系膜细胞 (MCs)结缔组织生长因子 (CT GF)表达的影响 方法 :分离培养SD大鼠肾小球系膜细胞。培养的系膜细胞中分别加入不同浓度的AngⅡ(10 -9、10 -7、10 -5mol/L) ,及 10 -7mol/LAngⅡ + 10 -5mol/LLosartan ,作用 72h后 ,采用反转录 聚合酶链反应 (RT PCR)技术测定MCsCTGFmRNA水平变化。 结果 :AngⅡ能促进MCsCTGFmRNA表达 ,且呈剂量依赖性 ;Losartan能部分降低AngⅡ对CTGFmRNA表达的诱导。 结论 :AngⅡ可能通过促进MCsCTGF的表达而促进了肾纤维化的进展 ;Losartan可以部分抑制AngⅡ对CTGFmRNA表达的诱导 ,从而可能有益于延缓肾纤维化的进展。

Connec tive tissue growth factor (CTGF) has been implicated in the process of renal fibro sis by stimulating renal cell proliferat ion and accumulation of extracellular ma trix.The effect might be influenced by a ngiotensin Ⅱ(Ang Ⅱ)or thus by angiotensi n Ⅱ receptor antagonist.In this study,we observe the effect of Ang Ⅱ and Losarta n (an Ang Ⅱ receptor antagonist) on the e x pression of CTGF and CTGF mRNA in cultur ed mesangial cells(MCs). MET HODOLOGY Mesangial cells of SD rats were isolated and cultured.Ang Ⅱ of different concentration(10 -9,10 -7,10 -5 mol/L) were added to the med i um for 72 hours to observe the influence of CTGF mRNA expression.Losartan of 10 -5mol/L and Ang Ⅱ of 10 -7 mol/L wer e added to the medium to observe the eff ects of Losartan on Ang Ⅱ stimulated CTG F mRNA expression. RESULTS Ang Ⅱ w as found to the expression of MCs CTGF m RNA in a dose-dependent manner.Expressio n of CTGF mRNA induced by Ang Ⅱ was part ially suppressed by Losartan at the conc cntration of 10 -7 mol/L. CONCLUSION Ang Ⅱ can enhanc e MCs expression of CTGF mRNA and thus is involved in the process of renal fibr osis.Losantan can partially suppress Ang Ⅱs effect of stimulating CTGF mRNA exp ression in cultured MCs,and thus implica tion in the treatment of renal fibrosis.