筛选控制果蝇心脏发育的基因

Screen on the Genes Involved in Heart Development of Drosophila

果蝇的早期心脏发育模式与脊椎动物的早期心脏发育模式具有惊人的相似性 ,研究果蝇心脏发育基因有助于揭示人体心脏发育机理和先天性心脏病发生机制。为了克隆和鉴定控制心脏发育的新基因 ,在化学诱变建立果蝇平衡致死系的基础上 ,用果蝇心脏组织特异抗体MabNo.3,对 310个平衡致死系进行免疫化学方法筛选 ,观察到有 6 3个致死系表现出心脏突变表型。分别将这些有心脏表型突变的品系与果蝇第 2和第 3染色体缺失系杂交 ,测定了 14个品系的遗传学位点 ,其中 7个品系在遗传学位点上有别于已报道的心脏发育控制基因。

Aim The developmental pattern of early heart in drosophila is remarkably similarity that in vertebrate. This reseach aims at approaching genes controlling human congenital heart disease. Methods In order to clone and identify new genes that are involved in drosophila heart development, after establishing the balanced lethal lines, 310 lethal lines were screened with Mab No.3 antibody expressed in heart cells of drosophila. Results 63 of them showedmutant phenotypes. Conclusions After examined with drosophila deficient stocks of chromosome 2 and 3,the cytological positions of 14 lethal lines have been determined. Candidate genes located in these cytological positions are searched and discussed.