细胞内游离钙离子在神经肽Y刺激血管平滑肌细胞增殖中的作用

Effects of the Intracellular Free Calcium Ion on the Proliferation of Cultured Vascular Smooth Muscle Cells Stimulated by Neuropeptide Y

为探讨细胞内游离钙离子在神经肽Y刺激血管平滑肌细胞增殖过程中的变化 ,以体外培养的血管平滑肌细胞模型为研究对象 ,运用激光共聚焦显微镜和免疫荧光组织化学技术 ,定量和动态观察了神经肽Y对血管平滑肌细胞内游离钙离子浓度与血管平滑肌细胞增殖间的相互关系。结果发现 ,神经肽Y组中血管平滑肌细胞的增殖活度 (MTT法 )和增殖细胞核抗原的表达较对照组明显增强 ;与此同时 ,神经肽Y作用下体外培养的血管平滑肌细胞内游离钙离子浓度也逐渐升高。此结果提示 ,神经肽Y刺激血管平滑肌细胞增殖的细胞内信号传导机制与神经肽Y提高细胞内游离钙离子浓度有关。

Aim To explore the role of intracellular free calcium in the proliferation of cultured vascular smooth cells (VSMC) stimulated by neuropeptide Y (NPY). [WT5”HZ]Methods The cultured VSMC of rat were used for experiments at passage 3 to 5. We applied the method of cellular culture, MTT colorimetric assay and quantitative immunocytochemistry through laser scanning confocal microscope (ACAS570) to detect the effect of NPY on the proliferation of VSMC. At the same time, the effect of NPY on the intracellular free calcium concentrations were also quantitatively detected in VSMC. Results It was observed that exposure of cultured VSMC to NPY could stimulate the proliferation of VSMC, the MTT OD values and expression of proliferating cell nuclear antigen (PCNA) increased in cultured VSMC respectively compared with control group (P<0.01).At the same time, the intracellular free calcium concentrations in VSMC were also increased by NPY. Conclusion The proliferation of cultured VSMC stimulated by NPY is mediated by intracellular free calcium in cultured vascular smooth muscle cells.