免疫胶体金与邻甲苯胺法联合检测粪便隐血应用价值初探

Discussion on combination of o-toluidine method,immune colloidal gold method in detection of fecal occult blood for clinical diagnosis of gastrointestinal bleeding

目的:通过两种方法联合检测粪便隐血,为临床消化道少量出血的诊断提供较为可靠的依据。方法:邻甲苯胺法、免疫胶体金法,分别对20例上消化道出血病人的粪便进行检测,对免疫的胶体金法阴性而邻甲苯胺法阳性标本进行分析。结果:化学法敏感性高,但特异性低;免疫法特异性好,但会出现假阴性。结论:在日常工作中化学法对粪便隐血只能起初筛作用,免疫法虽是较为可靠的方法,但对大便明显呈黑色或柏油样,而免疫胶体金法检测为阴性标本,用邻甲苯胺法作为免疫胶体金法的补充试验,进行联合检测,防止漏检,出现假阴性。

Objective：To discuss the detective methods through the combination of two methods in detection of fecal occult blood, it provides a reliable basis for clinical diagnosis of gastrointestinal bleeding. Methods ： o - toluidine method, immune colloidal gold method, respectively, were detected in stool of upper gastrointestinal hemorrhage in 20 eases, analysis of samples immune colloidal gold test nega- tive and positive o - toluidine method. Results ： Using chemical method has high sensitivity, but low specificity ; method of specific immuni- ty was good, but there would be false negative. Conclusion：In fecal occult blood in the immunochemical method first screen role in daily work, should be a reliable method, but it could black or tarry stool, and negative sample immune colloidal gold method, as a supplement of immune colloidal gold detection using o - toluidine method, joint detection, prevent omissions, false negative.