供核细胞来源对猪克隆胚胎发育的影响

Effects of Donor Cells with Different Sources on the Development of Porcine Cloned Embryos

为研究不同组织来源的供核细胞对猪体细胞核移植胚胎发育能力的影响,分别采用颗粒细胞、耳成纤维细胞和尾成纤维细胞作为供核细胞,移入体外成熟的猪去核卵母细胞中形成重构胚,培养后对比其卵裂率和囊胚率。结果表明:以耳成纤维细胞作为供核细胞的重构胚的囊胚率最高,为17.8%,显著高于颗粒细胞(11.4%)(P<0.05)。在卵裂率方面,两者无显著差别,分别为65.6%和61.9%(P>0.05)。猪尾尖成纤维细胞的发育能力最差,卵裂率仅为20.2%且未获得囊胚,与其它两组差异显著(P<0.05)。因此,耳组织来源的成纤维细胞作为供核细胞组织来源丰富且细胞易于传代和保存,以此为供核细胞的重构胚发育能力强,适合作为供核细胞。

In order to study the effect of donor cells with different sources on the ability of somatic cell nuclear transfer embryos development, the granular cells from COCs, fibroblast cells from ears or tails were obtained as materials,and they were transferred into enucleated porcine oocytes separately, the development of cloning embryos were compared. The results showed that the blastocyst rate was the highest about 17.8% when the ear fibroblast cells were used as donor cells,and the blastocyst rate was 11.4% when the granular cells were used. However the cleavage rate were 65. 6% and 61.9% respectively（P〈0. 05）, there had no significant difference between them. The cleavage rate was only 20.2% and no blastocyst was obtained when the tail fibro- blast cells were used as donor ceils, there had significantly difference with others（P〈0.05）. In conclusions, be- cause of the rich source and the convenience of extending and saving, fibroblasts derived from ears could be used as the donor cells for porcine nuclear transfer with strong development ability.