摘要
目的通过动物模型,探讨聚甲基丙烯酸甲酯(PMMA)诱导骨溶解对破骨细胞和骨溶解的影响,研究PMMA颗粒诱导假体周围骨质溶解的机制。方法选用8周龄BALB/c小鼠30只分为两组,小鼠背部注射无菌空气形成气囊后植入同系小鼠的颅骨,实验组小鼠注入PMMA颗粒,对照组注入等量生理盐水,14d后处死小鼠,对气囊组织及颅骨进行破骨细胞激活相关基因(RANK/RANKL)及自噬组织形态学检查。结果实验组抗酒石酸酸性磷酸酶染色(TRAP)阳性的破骨细胞(21.31±6.32)明显高于对照组(7.45±3.23),免疫组织化学结果显示实验组自噬相关蛋白微管相关蛋白1轻链3(LC3)、自噬基因(Beclin1)抗体染色评分等级明显高于对照组。实时定量聚合酶链反应显示实验组RANK基因mRNA含量(1.35±0.05)较对照组显著性增加(1.18±0.03)(P<0.05)。结论 PMMA诱导的自噬参与了骨溶解的形成。
Objective To explore the effects of polymethylmethacrylate（PMMA）-induced autophagy osteoclasts on bone dissolution animal models, and study the mechanism of PMMA particle-induced pyrophosphate osteolysis. Methods 30 8-week-old BALB/c mice were randomly divided into two groups,sterile air with back injection on mice to form airbag and homologous skulls were implanted into experimental group mice were injected with PMMA particles,the control group were injected with physiological saline. After 14 d,the mice were killed,osteoclast activation related gene（RANK / RANKL） and autophagy morphological examination of the the airbags tissue and the skull were detect. Results The tartrate-resistant enzyme staining（TRAP）-positive osteoclasts（21.31 ± 6.32）s of experiment group is significantly higher than that of the control group（7.45 ± 3.23）,immunohisto chemistry showed that autophytic protein microtubule-associated protein 1 light chain 3（LC3） and Beclin 1 antibody staining score level in experimental group was significantly higher than that of control group. RT-PCR showed that the RANK mRNA level（1.35 ±0.05） of experimental group was significantly increased（P〈0.05）. Conclusion The autophagy induce by PMMA is involved in the formation of osteolysis.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第34期4160-4161,4165,共3页
Chongqing medicine
