载多柔比星磁性四氧化三铁的制备及体外评价

Preparation and in vitro evaluation of doxorubicin-loaded magnetic iron oxide nanoparticles

本文采用水热法制备具有良好分散性的聚乙二醇(PEG)修饰的四氧化三铁(Fe3O4-PEG)纳米粒,通过透射电镜(TEM)、热重分析(TGA)等手段对其表征,考察其光热效果及对肿瘤细胞的杀伤作用,同时制备负载多柔比星(DOX)的Fe3O4-PEG纳米粒,研究其细胞毒性及联合治疗效果。结果表明,所制备的纳米粒分布均一,在水中的载药量达21%,体外释放具有pH敏感性。细胞毒性实验(MTT)结果显示,空白纳米粒具有较好的生物相容性,载药纳米粒具有较强的细胞毒性。联合治疗效果显示,光热治疗(PTT)可显著提高DOX的细胞杀伤作用。该研究表明,Fe3O4-PEG具有良好的光热效果及载药能力,可同时应用于肿瘤的光热及药物治疗,而这两种疗法具有潜在联合应用前景。

PEG-modified magnetic Fe304 （Fe304-PEG） nanoparticles were sythesized using a solvothermal reaction and characterized with transmission electron microscopy （TEM） and thermo gravimetric analysis （TGA）. The photothermal effect and photothermal destruction of cancer cells were evaluated. Then the doxorubicin loaded Fe304-PEG （DOX-Fe304-PEG） nanoparticles were prepared. The cytotoxicity and combined chemotherapy/photothermal therapy （PTT） effect were investigated. Uniform PEG coated Fe304 nanoparticles with particle size of 155 nm were obtained in the experiment. The loading and release of doxorubicin on Fe304-PEG were pH-dependent. The drug loading capacity in water was 21%. The results of MTT indicated a good biocompatiblity of Fe304-PEG nanoparticles and high cytotoxicity of DOX-Fe304-PEG. In combined therapy experiment, photothermal therapy demonstrated unambiguously enhanced chemotherapy efficacy. In conclusion, the obtained Fe304-PEG nanoparticles which exhibit good photothermal effect and drug loading capacity can be used for chemotherapy and photothermal therapy. The synergetic anti-tumor activity indicates the potential for the combined application of chemotherapy and photothermal therapy in cancer treatment.