血管细胞黏附分子-1蛋白在人脑胶质瘤组织内表达改变及其临床意义

Change of VCAM-1 protein expression in human brain glioma and its clinical significance

目的观察不同级别人脑胶质瘤组织中VCAM-1的表达情况,探讨其与脑胶质瘤病理分级的关系及临床意义。方法用免疫组织化学及蛋白免疫印迹技术,检测6例正常脑组织和30例脑胶质瘤组织中VCAM-1蛋白表达情况,比较各级别之间的表达差异,分析VCAM-1蛋白表达水平与脑胶质瘤病理级别的相关性。结果正常脑组织及各级别胶质瘤组织内VCAM-1阳性细胞率分别为:正常5.8%,Ⅱ级26.5%,Ⅲ级69.5%和Ⅳ级79.3%。高级别脑胶质瘤组织内,阳性细胞集中分布于肿瘤微血管周围。脑胶质瘤组织阳性细胞率明显高于正常脑组织(P<0.05);Ⅲ级和Ⅳ级恶性胶质瘤阳性细胞率显著高于Ⅱ级低级别胶质瘤(P<0.05);正常脑组织中VCAM-1表达量较低,胶质瘤组织中表达量则明显增多(P<0.05);Ⅲ级和Ⅳ级恶性胶质瘤内VCAM-1蛋白表达明显高于Ⅱ级胶质瘤(P<0.05);而Ⅲ级和Ⅳ级脑胶质瘤间,VCAM-1蛋白表达则无显著性差异。结论 VCAM-1蛋白在人脑胶质瘤组织中明显高表达,且与脑肿瘤病理级别相关,提示VCAM-1蛋白在脑胶质瘤恶性进展中可能发挥着重要作用。

Objective By observing the changes of vascular cell adhesion molecule-1 （VCAM-1） protein expression in human brain glioma tissue to determine its correlation with tumor pathological grade and clinical significance. Methods The distribution and changes of VCAM-1 protein expression in 6 normal brain and 30 brain glioma tissue were detected by using immunohistochemistry staining and Western blot analysis. Results The results of immunohistochemistry staining showed that the percentage of VCAM-1 positive cell number in normal brain tissue, grade II, III and IV brain glioma tissue were 5.8%, 26.5%, 69.5% and 79.3%, respectively. The VCAM-1 positive cells mainly distributed along the tumor capillaries in high pathological grade glioma. The number of VCAM-1 positive cells in glioma tissue was significantly higher than that of normal brain tissue （p〈0.05）. The higher number of VCAM-1 positive cells in grade III and IV glioma tissue was observed when compared with that of grade II glioma tissue（p〈0.05）, but no significant difference was found between grade III and grade IV glioma tissue （p=0.068）. Similarly, the Western blot analysis alse indicated that there were more VCAM-1 protein expressions in brain glioma tissue with the increase of the tumor grade level when compared with the less VCAM-1 expressed in normal brain tissue （p〈0.05）. In addition ,the VCAM-1 protein expressions in high pathological grade III and IV glioma tissue extensively increased compared with glioma II glioma tissue （p〈0.05）, but no significant difference in VCAM-1 protein expressions was observed between grade III and IV glioma tissue. Conclusion The significant increase of VCAM-1 protein expressions in brain glioma tissue positively correlated with the tumor pathological grades, suggesting an important role of VCAM-1 in the malignant progress of human brain glioma.