羟丙基β环糊精分子包合物促进姜黄素抑制肺腺癌A549细胞体外增殖和诱导凋亡

Hydroxypropyl beta cyclodextrin inclusion increased the inhibition effects of curcumin on A549 cell proliferation and induces the cell apoptosis in vitro

目的探讨羟丙基β环糊精分子包合是否促进姜黄素CUR抑制A549细胞增殖和诱导凋亡。方法以游离姜黄素为对照,用MTT法检测CUR羟丙基β环糊精分子包合物(CURCD)对A549细胞增殖的抑制;采用DAPI荧光染色,观察CURCD对A549细胞凋亡的影响;用流式细胞术检测CURCD对A549细胞周期、凋亡、活性氧和钙离子浓度、线粒体膜电位的影响。结果相对于游离CUR,CURCD对A549细胞增殖有显著抑制作用,显微镜下可见明显的形态改变,MTT检测显示细胞存活率降低,细胞生长被抑制在S/G2期,细胞凋亡率增加,细胞内活性氧和钙离子浓度上升,线粒体膜电位水平下降。结论羟丙基β环糊精分子包合可显著促进姜黄素抑制A549细胞增殖和诱导凋亡。

Objective The purpose of this study is to study the increased effects of curcumin （CUR） on A549 cell proliferation inhibition and induction apoptosis via including CUR into the hydroxypropyl beta cyclodextrin inclusion. Methods The inhibition rates were determined by MTT assay. Invert microscope was used to observe the changes of A549 cells after reated with CUR and CUR Hydroxypropyl beta cyclodextrin（CURCD） . Flow cytometric analysis was performed to detecte the cell cycle, apoptosis, reactive oxygen species, free Ca2＋ concentration, mitochondrial membrane potential. Results CURCD had effects on the proliferative activity of human pulmonary adenocarcinoma cell line A549 in a dose-dependent manner. Flow cytometric analysis indicated that cell cycles was blockaged at S/G2 phases, and cell apoptosis was obviously observed, reactive oxygen species and free Ca2＋ concentration were increased, mitochondrial membrane potential were decreased. The differences were remarkable between CUR and CURCD. Conclusion CURCD obviously increases the inhibition effects of CUR on A549 cell proliferation and induces its apoptosis.