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人工核酸酶用于基因组定点编辑的研究进展 被引量：4

Research progress in engineered nucleases for genome site-specific editing
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摘要 人工核酸酶(EN)技术,是利用设计并构建的核酸内切酶与靶DNA序列特异性结合,形成双链断裂(DSB),启动DNA修复通路,进行基因组定点编辑,目前已成功应用于多种细胞和生物体。 Engineered nucleases have been successfully applied to a variety of cells and organisms. The programmable engineered nucleases, with sequence-specific cleavage activity, enable a broad range of genome editing by inducing DNA double-strand breaks and thereby stimulate
作者 张殿宝 施萍 庞希宁
机构地区 中国医科大学基础医学院干细胞与再生医学研究室 中国医科大学附属第一医院全科医学教研室
出处 《基础医学与临床》 CSCD 北大核心 2013年第12期1634-1637,共4页 Basic and Clinical Medicine
基金 国家重点基础研究发展计划(2012CB518103) 辽宁省科学技术计划(2012225080) 沈阳市科学技术计划(F11-262-9-01)
关键词 人工核酸酶 基因组定点修饰 锌指核酸酶(ZFN) 类转录因子效应物核酸酶(TALEN) CRISPPR CAS 人工大范围核酸酶 engineered nucleases genome site-specific editing nuclease （TALEN） CRISPR./Cas engeered meganuclease DNA repair at specific genomic locations. zinc-finger nuclease （ZFN） transcription activator-like effector
分类号 R341 [医药卫生—基础医学]
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参考文献20

  • 1Rosenbloom KR,Sloan CA, Malladi VS, et al. ENCODEdata in the UCSC genome browser : year 5 update [ J]. Nu-cleic Acids Res,2013,41: 56 -63.
  • 2Gaj T,Gersbach CA, Barbas CF, et al. ZFN, TALEN,and CRISPR/Cas-based methods for genome engineering[J]. Trends Biotechnol, 2013,31 : 397 -405.
  • 3Mohr SE, Perrimon N. RNAi screening: new approaches,understandings,and organisms [ J]. Wiley Interdiscip RevRNA, 2012, 3; 145 -158.
  • 4Pan Y,Xiao L, Li AS, et al. Biological and biomedical ap-plications of engineered nucleases [ J]. Mol Biotechnol,2013,55: 54-62.
  • 5de Souza N. Primer: genome editing with engineered nucle-ases[ J]. Nat Methods, 2012, 9: 27.
  • 6Palpant NJ, Dudzinski D. Zinc finger nucleases: lookingtoward translation[ J]. Gene Ther, 2013,20: 121 - 127.
  • 7Carroll D. Genome engineering with zinc-finger nucleases[J]. Genetics, 2011, 188: 773 -782.
  • 8Bhakta MS,Henry IM,Ousterout DG, et al. Highly activezinc-finger nucleases by extended modular assembly [ J].Genome Res, 2013,23: 530 -538.
  • 9Joung JK, Sander JD. TALENs: a widely applicable tech-nology for targeted genome editing [ J] . Nat Rev Mol CellBiol, 2013, 14: 49 -55.
  • 10Mussolino C, Morbitzer R,Lutge F, et al. A novel TALEnuclease scaffold enables high genome editing activity incombination with low toxicity [ J]. Nucleic Acids Res,2011,39: 9283 -9293.

同被引文献23

  • 1NOH KM, HWANG JY, FOLLENZI A, et al. Repressor element- 1 si- lencing transcription factor (REST)-dependent epigenetic remodel- ing is critical to ischemia-induced neuronal death [J]. Proc Natl Acad Sci USA, 2012, 109 (16) : E962- 971. DOI: 10.1073/ pnas.1121568109.
  • 2ROCKOWITZ S, LIEN WH, PEDROSA E, et al. Comparison of REST cistromes across human cell types reveals common and con- text - specific functions [J]. PLoS Comput Bio|, 2014, 10 (6) : e1003671. DOI : 10.1371/journal.pcbi.1003671.
  • 3SMITH C, GORE A,YAN W, et al. Whole-genome sequencing anal- ysis reveals high specificity of CRISPR/Cas9 and TALEN-based ge- nome editing in human iPSCs [J]. Cell Stem Cell,2014, 15(1 ) : 12- 13. DOI: 10.1016/j.stem.2014.06.011.
  • 4LIN Y, FINE E J, ZHENG Z, et al. SAPTA : a new design tool for im- proving TALE nuclease activity [J]. Nucleic Acids Res, 2014, 42 (6) :e47. DOI: 10.1093/nar/gkt 1363.
  • 5HUANG P, XIAO A, TONG X, et al. TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish [J]. Methods,2014,69(1) :67-75. DOI:10.1016/j.ymeth.2014.02.010.
  • 6KELLIS M, WOLD B, SNYDER MP, et al. Defining functional DNA elements in the human genome [J]. Proc Natl Acad Sci USA,2014, 111(17):6131-6138. DOI : 10.1073/pnas.1318948111.
  • 7张余洋,张晓辉,张婵娟.利用人工锌指蛋白核酸酶进行植物基因定点突变和置换[J].中国生物工程杂志,2008,28(11):110-115. 被引量：2
  • 8李铁民,杜波.CRISPR-Cas系统与细菌和噬菌体的共进化[J].遗传,2011,33(3):213-218. 被引量：33
  • 9Jiyong Liu,Changqing Li,Zhongsheng Yu,Peng Huang,Honggang Wu,Chuanxian Wei,Nannan Zhu,Yan Shen,Yixu Chen,Bo Zhang,Wu-Min Deng,Renjie Jiao.Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy[J].Journal of Genetics and Genomics,2012,39(5):209-215. 被引量：45
  • 10Charles Ashton.Rapid and Cost-Effective Gene Targeting in Rat Embryonic Stem Cells by TALENs[J].Journal of Genetics and Genomics,2012,39(6):275-280. 被引量：21

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基础医学与临床
2013年 第12期

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