补肾中药复方与跑台训练联合干预对模拟失重大鼠骨密度及骨代谢指标的影响

Chinese Medicine Compounds Combined with Treadmill Training Improves the Bone Mineral Density and Bone Metabolism of Rat under Simulated Weightlessness

目的:研究补肾中药复方与跑台训练联合干预对模拟失重大鼠骨密度和骨代谢指标的影响。方法:9周龄Wistar雄性大鼠40只,随机分为对照组、失重对照组、中药干预组、跑台训练干预组、中药+跑台训练联合干预组5组,每组8只。采用后肢悬吊方法制备模拟失重大鼠模型,悬吊4周。悬吊结束后,分别给予补肾中药复方、跑台训练及中药+跑台训练联合干预措施,干预周期均为8周。8周后,测试各组大鼠左侧股骨骨密度和血清骨代谢指标血钙、碱性磷酸酶、骨钙素和抗酒石酸酸性磷酸酶5b。结果:失重对照组大鼠血钙和抗酒石酸酸性磷酸酶5b显著高于正常对照组(P<0.01),而血清碱性磷酸酶和骨钙素差异无统计学意义(P>0.05),骨密度显著低于正常对照组(P<0.01)。中药干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.05,P<0.01),而血清碱性磷酸酶和骨钙素差异无统计学意义(P>0.05),骨密度显著高于失重对照组(P<0.01)。跑台干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.05,P<0.01),血清碱性磷酸酶和骨钙素显著高于失重对照组(P<0.01),骨密度显著高于失重对照组(P<0.01)。中药+跑台训练联合干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.01),血清碱性磷酸酶和骨钙素显著高于失重对照组(P<0.01)。联合干预组血钙和抗酒石酸酸性磷酸酶5b显著低于中药干预组和跑台训练干预组(P<0.05,P<0.01),而血清碱性磷酸酶和骨钙素与各单一干预组比较差异无统计学意义,骨密度显著高于中药干预组和跑台训练干预组(P<0.01)。结论:补肾中药复方和跑台训练联合干预有效抑制模拟失重大鼠骨吸收,增强骨形成,升高骨密度,其效果优于单纯中药复方或跑台训练干预。

Objective The purpose of this study is to investigate the effect of Chinese mealcm~ compounds for reinforcing kidney in combination with treadmill training on the bone mineral density and bone metabolism of rats under simulated weightlessness. Methods 40 8-week old male Wistar rats were randomly divided into normal control group,weightlessness control group,Chinese medicine intervention group ,treadmill training group, and weightlessness plus Chinese medicine group. Weightlessness of the rats was simulated through 5-week hind limbs suspension. All the intervention on rats lasted for 8 weeks. Then,the femoral bone mineral density and blood serum calcium,alkaline phosphatase, osteocalcin and tartrate resistant acid phosphatase of each group, were tested. Results As compared to the control group,blood calcium and tartrate resistant acid phosphatase 5b in the weightlessness control group significantly increased（P 〈 0.01 ）,and bone mineral density decreased significantly（P 〈 0.01 ）,but no significant differences in serum alkaline phosphatase and osteocalcin （P 〉 0.05）. Comparing to the weightlessness control group,the blood calcium and tartrate resistant acid phosphatase 5b in Chinese medicine intervention group significantly reduced （P 〈 0.05 and P 〈 0.01 ）, and the bone mineral density increased significantly （P 〈 0.01）,but no significant differences in serum alkaline phosphatase and osteocalcin （P 〉 0.05）. Comparing to the weightlessness control group,the blood calcium and tartrate resistant acid phosphatase 5b significantly reduced,the serum alkaline phosphatase and osteocalcin significantly increased （P 〈 0.05 ,P 〈 0.01）,and the bone mineral density increased significantly （P 〈 0.01 ）in treadmill training group. Comparing to the weightlessness control group,the blood calcium and tartrate resistant acid phosphatase 5b significantly decreased, and the serum alkaline phosphatase and osteocalcin significantly increased （P 〈 0.01）in the weightlessness plus Chinese medicine intervention group. Compared to both Chinese medicine intervention group and treadmill training group,the blood calcium and tartrate resistant acid phosphatase 5b decreased significantly （P 〈 0.05 ,P 〈 0.01 ）,and the bone mineral density increased significantly （P 〈 0.01）in the weightlessness plus Chinese medicine group,but there was no significant difference in blood calcium and tartrate resistant acid phosphatase 5b between Chinese medicine intervention group and treadmill training group （P 〉 0.05）. Conclusion The joint intervention of Chinese medicine compounds for reinforcing kidney and treadmill training effectively inhibits the bone resorption and improves bone formation in rat under simulated weightlessness.