6-羟基多巴胺单侧两点注射法构建的帕金森病模型鼠

Establishment of rat models of Parkinson's disease by unilateral two-point injection with 6-hydroxydopamine

背景:帕金森病动物模型的建立对帕金森病的临床、基础实验研究中有重要作用,同时其稳定性也直接影响到研究的结果。目的:评价6-羟基多巴胺单侧两点注射法构建的帕金森病模型大鼠行为及病理变化。方法:SD大鼠62只随机分为实验组50只,正常组12只,采用脑内立体定向,将6-羟基多巴胺注入实验组大鼠右侧黑质致密部和中脑腹侧被盖区以建立帕金森病模型,观察大鼠行为变化、酪氨酸羟化酶及脑内多巴胺含量改变。结果与结论:2周后实验组经阿朴吗啡诱导后,有22只向左侧旋转速度>7 r/min,2周与4周之间大鼠的旋转圈数差异无显著性意义(P>0.05)。模型组中酪氨酸羟化酶及脑内多巴胺含量明显减少。说明应用6-羟基多巴胺单侧两点注射可成功建立行为及病理相似的帕金森病大鼠模型。

BACKGROUND： The establishment of animal models of Parkinson＇s disease plays an important role in clinical and basic experiments of Parkinson＇s disease, and its stability directly affected study results. OBJECTIVE： To evaluate the behavior and pathological changes of rats with Parkinson＇s disease established by unilateral two-point injection with 6-hydroxydopamine. METHODS： A total of 62 Sprague-Dawley rats were randomly assigned into experimental group （n=50） and normal group （n=12）. Using stereotatic technique, 6-hydroxydopamine was injected into the right substantia nigra compact part and midbrain ventral tegmental area to establish models of Parkinson＇s disease in the experimental group. Behavioral changes of rats, tyrosine hydroxylase and dopamine content were observed. RESULTS AND CONCLUSION： At 2 weeks, after induction with apomorphine, left rotation speed 〉 7 r/min was observed in only 22 rats of the experimental group. No significant difference in the number of rotation between 2 and 4 weeks was detected （P 〉 0.05）. Tyrosine hydroxylase and dopamine content significantly reduced in the model group. Results indicated that unilateral two-point injection of 6-hydroxydopamine could be used to successfully establish rat models of Parkinson＇s disease with similar behaviors and pathologies.