房山封闭群小型猪微卫星位点的测定

Study on DNA polymorphisms of microsatellite locus in Fangshan closed colony miniature pigs

目的依据北京市地方标准DB11/T828.3-2011,在DNA水平上对房山封闭群小型猪进行种群遗传质量评价与分析。方法按照北京市地方标准DB11/T828.3-2011规定,利用25对微卫星引物,对22头房山封闭群小型猪进行PCR检测,利用群体遗传分析软件Popgen32对所得结果进行处理。结果 25对引物中,4对引物经反复调整PCR反应条件不能得到稳定扩增结果,其余21对引物得以稳定扩增;共得到91个有效等位基因型,平均多态性信息含量(PIC)为0.4630,平均杂合度为0.5331。同时,SWr1008位点上的产物与DB11/T828.3-2011标准有所差异;标准对SW271位点所在染色体报道也与美国农业部官网等数据库不同。结论房山封闭群小型猪群体具有一定的遗传稳定性,符合封闭群动物的遗传结构特征。

Objective To analyze and evaluate the genetic quality of Fangshan closed colony miniature pig population in DNA level according to DB11/T828.3-2011. Methods According to the local standard DB11/T828.3- 2011, 25 pairs of microsatellite primers were used in 22 Fangshan closed colony miniature pigs for polymerase chain reaction. Popgen32 was used for result handler. Results Among 25 pairs of primers, 4 of that were not able to get stable amplification results, even when changing reaction condition; 21 pairs of primers were able to get stable amplification results. There are 91 valid allelotype in 21 mierosatellite locus. The average PIC is O. 4630, and the average heterozygosity is 0. 5331. What＇ s more, products of SWr1008 is different from that of DB/T828.3-2011. And the chromosome position of SW271 in DB/T828.3-2011 and http：//www, marc. usda. gov also exists differences. Conclusion Fangshan closed colony miniature pigs with genetic stability satisfy the genetic standard of closed colony animal.