摘要
目的:研究基于自动分层算法的超高分辨率谱域OCT(UHR-OCT)用于角膜上皮厚度测量的重复性。方法:使用自行搭建的UHR-OCT系统采集18例健康受试者(年龄23.2±0.9岁)左眼的图像,应用一种基于轴向梯度信息和最短路径的分层算法对得到的SD-OCT图像进行边界探测获取角膜上皮厚度。结果:自动与手动的上皮分层的位置有很好的相关性(P>0.05,配对t检验)。自动算法获取的6mm范围平均上皮厚度为52.1±2.1μm,范围从50.3±1.8μm到53.2±2.4μm,与手动分层之间的角膜上皮厚度差异为0.4±0.3μm。UHR-OCT自动算法重复测量上皮厚度的组内相关系数ICC和可重复性系数CoR分别为0.90±0.05,1.44±0.01μm。结论:对于超高分辨率OCT,自动分层方法在角膜上皮厚度测量中有着很好的准确性和可重复性。
AIM:To assess the repeatability of thickness profiles of corneal epithelium produced by aautomatic hierarchic algorithm that was applied to images obtained by a custom-built ultra-high resolution OCT ( UHR-OCT ) system.
METHODS:Eighteen left eyes of 18 subjects (23.2±0.9) years;mean age±standard deviation) were imaged twice on non-consecutive days by a custom-built ultra-high resolution OCT ( UHR -OCT ) system. A hierarchic algorithm based on the axial gradient information and a shortest path search was developed to measure the corneal epithelial thickness profiles from the SD-OCT images.
RESULTS: There was good correlation between theautomatic and manual hierarchy positions of the epithelium ( p 〉0.05, paired t -test ). The average epithelial thickness of the 6mm central corneal region determined by the automatic hierarchic algorithm was 52.1±2.1μm, ranging from 50.3 ±1.8μm to 53.2 ±2.4μm along the horizontal meridian. The epithelial thickness differences between automatic and manual hierarchy by the UHR-OCT systems were 0.4 ±0.3μm.The intraclass correlation coefficients ( ICC ) and coefficients of repeatability ( CoR ) with repeated UHR - OCT measurements for epithelial thickness were 0.90±0.05 and 1.44±0.01μm respectively.
CONCLUSION: Regarding UHR -OCT system, the custom -developed automatic hierarchy has good repeatability and accuracy for measuring the thickness of corneal epithelium.
出处
《国际眼科杂志》
CAS
2013年第12期2475-2478,共4页
International Eye Science
基金
浙江省医药卫生科技计划项目(No.2013KYA132)
温州市科技局项目(No.Y20110058
No.Y20110103)~~
关键词
自动分层
超高分辨率OCT
角膜上皮
automatic hierarchy
ultra- high resolutionOCT
corneal epithelium