Saposin C促进PC3细胞p27^(KIP1)蛋白泛素化降解的研究

Saposin C promotes p27^(KIP1) protein ubiquitination and degradation in PC3 cells

目的:探讨Saposin C促进PC3细胞抑癌基因p27KIP1蛋白泛素化降解,刺激细胞增殖,以及与PI3K/AKT信号通路的关系。方法:利用含有Saposin C的真核表达载体转染PC3细胞,检测Saposin C对PC3细胞Skp2、抑癌基因p27KIP1蛋白水平的调节以及对细胞增殖的影响。结果:Saposin C通过激活PI3K/AKT信号途径上调细胞内Skp2的蛋白含量,下调抑癌基因p27KIP1的蛋白水平,其机制是加速p27KIP1的蛋白降解,促进PC3细胞增殖。结论:Saposin C在PC3细胞中通过激活PI3K/AKT信号途径促进抑癌基因p27KIP1的蛋白泛素化降解,刺激细胞增殖。

Objective：To investigate the effect of Saposin C on stimulation of p27KIPI degradation and stimulation of cell proliferation via PI3K/AKT signal pathway in PC3 cells. Methods：The eukaryotic expression vectors were transfected into PC3 cells containing Saposin C. Effects of Saposin C on Skp2 ,tumor suppressor gene p27KIPI protein level regulation and on PC3 cells proliferation were investigated. Results： Saposin C could upregulate Skp2 protein level and down regulate tumor suppressor gene p27KIPI protein level, its way is to accelerate the degradation of p27KIPI protein. Saposin C could also promote the proliferation of PC3 cells through activating PI3K/AKT signaling pathway. Conclusion：Saposin C could down regulate level of tumor suppressor gene p27 KIPI , accelerate its protein ubiquitina- tion and degradation via PI3K/AKT signaling pathway in PC3 cells.