NDRG2对A549肺癌细胞增殖的抑制作用

The depression effect of NDRG2 on the proliferation of lung cancer cell A549

目的:探讨NDRG2(N-myc downstream regulated gene 2)对于肺癌细胞系增殖的抑制作用。方法:将NDRG2基因转染进肺癌细胞系A549,采用G418筛选出稳定高表达NDRG2的亚克隆细胞系A549/NDRG2-flag并将仅转染载体的A549/pcDNA3.1(+)作为阴性对照。Western blot检测NDRG2在转染细胞中的表达水平。MTT、平板克隆形成试验检测转染前后细胞增殖作用的差异,流式细胞仪检测转染后细胞的周期分布状态。结果:成功构建高表达NDRG2的亚克隆细胞系A549/NDRG2-flag。证实了NDRG2的高表达能够抑制肺癌细胞系的增殖(P<0.01),使肺癌细胞的细胞周期阻滞在G0/G1期。结论:NDRG2高表达后能够有效抑制肺癌细胞系A549增殖并阻滞细胞周期滞留在G0/G1期。

Objective：To explore the depression effect of gene NDRG2 （ N - myc downstream regulated gene 2） transfection on the proliferation in lung cancer. Methods ： pcDNA3.1 - NDRG2 - flag and eukaryotic expression vec- tors pcDNA3.1 （ ＋ ） were transfected into lung cancer cell line A549 by Liposome 2000. The subelone cell line A549/NDRG2- flag expression NDRG2 stably and highly were obtained by persistent G418 selection. NDRG2 ex- pression levels in the transfected cell lines were detected by Western blot and the proliferation activity of the cell lines was detected by MTr and MTr plate colony formation. The cell cycle was detected by flow eytometry. Results：The subelone cell lines A549/NDRG2 - flag were successfully selected because of the ability of expressing NDRG2 stably and highly. The high expression of NDRG2 induced weak proliferation of lung cancer cell line （ P 〈 0.01 ） and the cells were arrested at G0/G1 phase （P 〈 0.01 ）. Conclusion：Over- expression of NDRG2 in A549 cells effectively inhibits cell proliferation and induces cell cycle arrest.