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培美曲塞联合顺铂对人结肠癌SW480细胞增殖和凋亡的影响

Effects of pemetrexed combined with cisplatin on proliferation and apoptosis of human colon cancer cells
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摘要 目的探讨培美曲塞联合顺铂对人结肠癌细胞SW480增殖和凋亡的影响。方法 SW480细胞单用培美曲塞(A组)、顺铂(B组)或二药联用(AB组)处理,采用MTT法检测细胞增殖抑制率,流式细胞术检测细胞周期及凋亡率,蛋白质印迹法检测增殖细胞核抗原(PCNA)和凋亡相关蛋白B细胞淋巴瘤/白血病2(Bcl-2)表达。结果培美曲塞和顺铂均呈剂量依赖性抑制SW480细胞的增殖,二药联用作用更为明显。AB组(77.8±2.3)%的SW480细胞被阻滞于G1期,高于A组的(64.9±2.2)%和B组的(66.3±1.1)%(P<0.05)。AB组SW480细胞凋亡率为(21.8±2.3)%,高于A组的(8.5±0.5)%和B组的(11.2±2.1)%(P<0.05)。三组SW480细胞中PCNA和Bcl-2蛋白表达量均较处理前下降(P<0.05)。结论培美曲塞和顺铂均可抑制结肠癌细胞SW480的增殖,并促进其凋亡;二药联用作用更明显。 Objective To investigate the effects of pemetrexed combined with cisplatin on the proliferation and apoptosis of SW480 cells. Methods The SW480 cells were treated with pemetrexed (group A) or cisplatin(group B) alone or in combination(group AB). The growth, apoptosis and cell cycle of SW480 cells were examined by MTT assay and flow cytometry. The expressions of proliferating cell nuclear antigen(PCNA)and cell apoptosis-associated protein Bcl-2 were detected by Western blot. Results Pemetrexed and cisplatin suppressed dose-dependently the growth of SW480 cells, which was more when combined use of both drugs. The percentage of the cell cycle blocked at G1 phase in group AB was (77. 8±2.3) % ,which was higher than (64.9±2.2) % in group A or (66.3±1.1)% in group B(P〈0. 05). The apoptosis rate of SW480 cells in group AB was (21.8±2.3)%, which was higher than (8.5±0. 5)% in group A or (11.2±2. 1)% in group B(P〈0. 05). The expressions of PCNA and Bel-2 in three groups were lower after treatment than those before (P〈0. 05). Conclusion Pemetrexed and cisplatin can inhibit cell proliferation and promote the apoptosis of SW480 cells, which is better when combined use of both drugs.
作者 杨金虎 任峰
出处 《江苏医药》 CAS 北大核心 2013年第23期2820-2822,共3页 Jiangsu Medical Journal
关键词 培美曲塞 顺铂 人结肠癌SW480细胞 Pemetrexed Cisplatin Human colon carcinoma SW480 cells
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参考文献6

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