重组HIV-1腺病毒伴随病毒的构建及表达

Construction and expression of recombinant adeno associated HIV 1 virus

目的 构建带有HIV 1gag或 gp12 0基因的重组腺病毒伴随病毒AAV HIV gag或AAV HIVgp12 0。方法 共转染法获取重组AAV HIV ;免疫酶法检测病毒滴度。结果 测定制备的重组腺病毒伴随病毒AAVgag42 ,AAVgp42 ,AAVgpRj6 ,病毒滴度在 10 4～ 10 5 范围。结论 构建了带有 gag和gp12 0基因的重组AAV HIV ,可以感染真核细胞并有较高的表达 ,可以用于做靶细胞 ,为发展新型的HIV

Objective Plasmid of AAV vector with HIV 1 gag and gp 120 were constructed respectively. Methods The recombinant AAV HIV gag and gp120 viruses were obtained by co transfection of AAV HIV plasmid and Adenovirus 5 in 293 cells. Results The recombinant AAV HIV virus expressed well and the titer of the recombinant viruses were between 10 4 10 5. Conclusion The recombinant AAV HIV virus can be used to infect the target cells for detection of cellular immunity and for vaccine studies.