人乳头瘤病毒与P53协同致膀胱移行细胞癌关系的研究

The study on relation of human papillomavirus and P53 expression with bladder transitional cell carcinoma

目的 研究人类乳头瘤病毒 (HPV) 6、11、16和 18型及P5 3与膀胱移行细胞癌的关系。方法 采用聚合酶链反应 (PCR)方法检测了 75例膀胱移行细胞癌组织中HPV的感染 ,免疫组化SP法检测P5 3蛋白表达情况。结果 膀胱移行细胞癌组织中HPV6、11、16和 18的阳性率分别为 6 .7%(5 75 ) ,5 .3 % (4 75 ) ,33 .3 % (2 5 75 )和 6 .7% (5 75 )。低危型HPV(6或 11)阳性率为 9.3% (7 75 ) ,高危型HPV(16或 18)阳性率为 34.7% (2 6 75 )。同一膀胱癌组织中两种以上 (包括两种 )HPV亚型感染 8例 ,占 10 .6 %。HPV6、16和 18型之间感染阳性率在肿瘤有无转移组中差异显著 (P <0 .0 5 ) ,HPV16、18的阳性率在肿瘤病理分级中差异有极显著性 (P <0 .0 1)。HPVDNA型别的分布在膀胱癌中以 16型为主。P5 3蛋白在膀胱移行细胞癌组织中的阳性表达率为 44 %。

Objective To study the relationship between the bladder transitional cell carcinoma and the human papillomavirus (HPV) types 6,11,16,18 and P53.Methods We used polymerase chain reaction (PCR) to test HPV type 6,11,16 and 18 infection from 75 cases of bladder cancer, and used immunohistochemisty method to detect P53 expression. Results The positive rates of HPV types 6,11,16 and 18 were 6.7 %, 5.3 %, 33.3 % and 6.7 %,respectively. The positive rate of low risk HPV type (6/11) was 9.3 %(7/75), and that of high risk HPV types (16/18) was 34.7 % (26/75). There were 8 cases with two or three HPV types mixed infection,accounting for 10.6 %, HPV types 6,11,16,18 in tumor metastasis group were significantly higher than that in non metastasis group. The positive rates of HPV 16,18 types in G3 were significantly higher than that in G1 and G2. HPV type 16 was the main type in bladder cancer. Positive rate of P53 expression was 44%. Conclusion These results implicated that the occurrence and development of bladder transitional cell carcinoma may be closely related to the high risk HPV type infection and P53 expression.