杂草对芳氧苯氧丙酸类(APPs)除草剂的抗性分子机理研究进展

Research progress on molecular mechanism of resistant weeds to aryloxyphenoxypropionate herbicides

芳氧苯氧丙酸类(aryloxyphenoxypropionates,APPs)除草剂是一类广泛使用的乙酰辅酶A羧化酶抑制剂,可高效专一抑制禾本科杂草的乙酰辅酶A羧化酶(acetyl-coenzyme A carboxylase,ACCase)。目前已出现大量抗芳氧苯氧丙酸类除草剂的禾本科杂草,其抗性大多由叶绿体乙酰辅酶A羧化酶的羧基转移酶(carboxyltransferase,CT)功能域中的氨基酸突变引起。在所有已发现的氨基酸突变中,最引人关注的是第1 781位(对应大穗看麦娘Alopecurus myosuroides质体ACCase的氨基酸残基位置)异亮氨酸到亮氨酸的单点突变,该特定位置形成亮氨酸会导致某些杂草对APPs类除草剂产生抗性。综述了乙酰辅酶A羧化酶CT功能域的研究进展及杂草对APPs类除草剂的抗性分子机理,探讨了杂草对APPs类除草剂抗性分子机理研究中存在的问题,以期为进一步深入研究APPs类除草剂的抗性机制提供参考。

Aryloxyphenoxypropionate （APP） herbicides can effectively inhibit plastid acetyl-coenzyme A carboxylase （ACCase） of gramineous plant, and have been widely used to control gramineous weeds in the field. Gramineous weeds resistant to APP herbicides have become widespread after the long-term use of ACCase inhibitors, which are mostly caused by amino acid mutation in chloroplast ACCase carboxyltransferase （CT） domain. Among all of the amino acid mutations, Ile1781/Leu mutation is the most interesting one, which is found in almost all the resistant weeds. A Leu at the specific location may lead to APPs herbicides resistance in some gramineous weeds. The research progress of ACCase CT domain and molecular mechanism of resistance to APPs herbicides were summarized in this paper in order to provide useful information and references for further study of APPs herbicides resistance. Potential problems in the study of molecular mechanism of weeds resistance to APPs herbicides were also discussed.