乐至黑山羊催乳素受体基因cDNA克隆及序列分析

Cloning and sequence analysis of Lezhi black goat prolactin receptor cDNA

根据GenBank中绵羊催乳素受体(PRLR)基因序列(AF041257.1)设计1对引物,以乐至黑山羊脑垂体总RNA为模板,通过RT-PCR技术对乐至黑山羊PRLR基因cDNA克隆测序和序列分析.结果表明:扩增出的乐至黑山羊PRLR基因cDNA序列长为1743bp,编码581个氨基酸.乐至黑山羊与绵羊、牦牛、欧洲牛、野猪和人的核苷酸同源性分别为:98.34%、94.67%、94.27%、77.48%、74.33%.以核苷酸序列构建分子进化树,乐至黑山羊先与绵羊聚为一类,再与欧洲牛和牦牛聚为一类,而后与野猪聚为一类,最后与人聚为一类.

The primers are designed according to the GenBank gene sequence of sheep PRLR （AF041257.1）. Total RNA is extracted from the pituitary of the Lezhi black goat and the PRLR gene cDNA is obtained by the reverse transcription PCR （RT-PCR） and then the sequence is analyzed. The result demonstrates that the size of Lezhi black goat PRLR gene cDNA is 1743bp which encodes 581 amino acids. The homologies of nucleotide sequences of PRLR gene between the Lezhi black goat and sheep, yak, cattle, pig and human are 98.34%, 94.67%, 94.27%, 77.48% and 74.33%, respectively. According to the nucleotide sequence to construct molecular phylogenetic tree, the result indicates that Lezhi black goat and sheep, yak and cattle assemble separately, and then assemble with pig, and finally assemble with human.