+G_Z暴露对大鼠脑热休克蛋白-70表达水平的影响

Effect of +G_Z exposures on expression of heat shock protein 70 in the rat brain

目的 探讨 +GZ暴露条件下大鼠脑中热休克蛋白 - 70 ( HSP70 )的变化及其在 +GZ所致脑损伤中的作用。 方法 10 0只大鼠 ,随机分为 4组 ,每组 2 5只 ,一组作为空白对照 ,其余三组分别进行 +2 GZ,+6 Gz和 +10 GZ暴露 ,峰值作用时间 3min,加速度增长率 1G/s。然后 ,分别于暴露后的 6 h、1d、2 d、4d和 6 d麻醉大鼠 ,心脏灌注后迅速取脑组织 ,用 West blot法比较 HSP70的变化情况。 结果 +GZ暴露后 6 h HSP70开始升高 ,1d达到高峰 ,至 6 d时基本恢复正常。不同 +GZ暴露之间进行比较可以看出 ,+6 GZ组 HSP70表达最高 ,+10 GZ最少。 结论 +GZ暴露对大鼠脑HSP70表达水平有显著的影响。

Objective To explore the effect of +G Z exposures on expression of heat shock protein 70(HSP70) in the rat brain. Methods One hundred SD rats were randomly divided into 4 groups: 1 control group, and 3 experimental groups, which underwent +2 G Z, +6 G Z, +10 G Z exposures for 3 min respectively. The expression levels of HSP70 in rat brain were measured by West blot 6 h, 1 d, 2 d, 4 d and 6 d after +G Z exposures. Results As compared with control, the expression levels of HSP70 began to raise 6 h after +G Z exposure, up to the peak value 1 d later, and returned to normal after 6 d. As to the degree of change of HSP70 expression following various +G Z exposures, the most obvious elevation was observed after +6 G exposure, and the least after +10 G exposure. Conclusion Increase of HSP70 expression in rat brain can be induced by +G Z exposures. It may play a role in protecting the brain from injury following +G Z exposures.