新生期重复吸入七氟烷对大鼠行为学的远期影响

Long-term effects of repeated inhalation of sevoflurane on behavioristics in neonatal rats

目的探讨新生期重复吸人七氟烷对大鼠幼年期、成年期的行为学影响。方法7日龄SD大鼠（n=24），随机分为2．6％七氟烷组（n=8，sev2．6组）、1．50A七氟烷组（n=8，sevl．5组）、对照组（n=8，con组）。分别于出生7、14、21d（P7，P14，P21）吸人2．6％、1．5％七氟烷及运载气体1h，于P28、P29行可视平台实验，P32～P36及P91～P95行定位航行和空间探索实验。结果1）定位航行实验逃避潜伏期幼年期：sev2．6组和sevl．5组较con组延长（P〈0．05），其中sevl．5组于P32、P36显著延长（P〈0．01）；sev2．6组较sevl．5组延长（P〈0．05）。成年期：sevl．5组较con组延长（P〈0．05）。2）空间探索实验成年期sevl．5组平台区进入次数较con组减少（P〈0．05）。结论新生期重复吸人七氟烷损害幼年大鼠的陈述性记忆功能，且有浓度依赖性。1．5％七氟烷陈述性记忆的损害有两个峰值，可延长至成年，并损害成年期空间联想和回忆探究能力。

Objective To investigate the effects of repeated inhalation of sevoflurane anesthesia in the neonatal period on capability of behaviorlstics in juvenile and adult period. Methods 7 clay old SD rats （n= 24） were randomly divided in to 3 groups（n=8 each） ：2.6sevoflurane group（sev2.6） ,1.5%sevoflurane group （sevl. 5） and control group （con）. The rats were exposed to 2.6% sevoflurane or 1.5% sevoflurane in carrier gas （sev2.6,sevl. 5） or simply carrier gas （1 L/min of O2 and 1L/rain of air） without any inhalational anesthetic （con） for I h at 7,14 d and 21 d after birth （P7,P14,P21）. The cued trials was performed at P28 and P29, while the place trials and probe trials were performed in P32 - P36 and P91 P95. Results 1）In the juvenile place trials,the escape latency of group sev2.6 and sevl. 5 were longer than group con （P 〈0.05） ,and the escape latency of group sevl. 5 were significantly longer than the group con at P32,P36 （P〉0.01）. The escape latency of group sev2.6 was longer than sevl. 5 （P〈0.05）. In the adult place trials,the escape latency of sevl. 5 were longer than the group con （P〈0.05）. 2）In the adult probe trials, times of crossing target area in sevl. 5 were shorter than group con （P〈0. 05）. Conclusion Intermittent repeat inhalation of sevoflurane anesthesia during the neonatal period may injured the declarative memory in juvenile period,and exist concentration dependence. 1.5 % of sevoflurane on declara tive memory function damage may show two peak period, and extended to adulthood, while injured the space association abil- ity and recall inquisition ability in adulthood.