用绿色荧光蛋白标记的细菌研究鱼体吸收颗粒抗原的部位

Application of green fluorescent protein marked bacteria to investigate the site of antigen uptake in Carassius auratus ibelio

用绿色荧光蛋白标记的嗜水气单胞菌 4332株 (Ah4332 GFP)菌液高渗浸泡和直接浸泡异育银鲫 ,研究鱼体对颗粒抗原的吸收。经两种方法处理的鱼 ,在整个实验过程中显示 ,鱼鳃的标记菌检出量均高于皮肤和肠道中的标记菌 ,证实鳃是抗原吸收的主要部位。另外 ,鱼体经高渗处理 ,鳃和皮肤可检出的标记菌明显增加。在肠道中 ,除浸泡 6 0min外 ,浸泡 10min、30min及 12 0min的细菌检出量也明显增加。

The uptake of granular antigen was examined in Carassius auratus ibelio after bath administration of GFP marked Aeromonas hydrophila strain 4332(Ah4332 GFP ) by hyperosmotic infiltration (HI) and direct immersion (DI). Much more marked bacteria had been found in gills than that in skin and gut in both treatments throughout the experiment. Quantitative analyses indicated that the main site of antigen uptake were the gills in both HI and DI treatments. In addition, the amount of marked bacteria in gills and skin had increased obviously after hyperosmotic treatment, and gut also had increased bacteria during experiment of 10min, 30min and 120min with the exception of 60 min after immersion. It showed that hyperosmotic treatment facilitated antigen entrance in fish.