间接Dot-ELISA检测EDS-76病毒抗原的研究

Study on the use of indirect Dot-ELISA in examining EDS 76 virus antigens

本研究成功地建立了间接酶联免疫吸附试验 (Dot- EL ISA )用于检测 EDS- 76病毒抗原的标准化程序。对纯化EDS- 76病毒抗原的最低检出量为 lng/ Dot,其敏感性约为 HA的 15 .6倍。 EDS- 76阳性鸡血清特异性阻断试验及交叉反应试验证明 ,该法对 EDS- 76病毒抗原的检测具有特异性。试验结果表明 ,间接 Dot- EL ISA检测 EDS- 76病毒抗原 ,具有敏感性高、特异性强、重复性好、经济、方便、快速等优点 。

A standard procedure was successfully established to examine EDS 76 virus antigens with the indirect dot enzyme linked immunosorbent assay (Dot ELISA).Pure EDS 76 virus antigens were detectable at a minimum of l ng/dot, and 15.6 times as sensitive as HA. Distinctive obstruction and cross response tests with EDS 76 positive chicken serum indicated that this method was specific to EDS 76 virus antigens. It was concluded that examination of EDS 76 virus antigens with the indirect Dot ELISA is highly sensitive, specfic, repeatable, economical, convenient and quick, and applicable to examination of large sized samples.