Pot 2在稻瘟病菌群体结构分析中的应用

Application of Pot 2 on the Analysis of Population Structure of Magnaporthe grisea

利用稻瘟病菌的一段倒位重复序列Pot2设计的一对引物直接对稻瘟病菌的DNA进行PCR扩增。根据带型相似性大于 80 %为度可将国际水稻所一个稻瘟病圃上的 31 0个菌株划分 6个宗亲群 (lineage)。这些依分子标记划分的宗亲群与品种的遗传背景有密切关系 ,而且各宗亲群在水稻不同生育期有不同的波动方式。

DNA of 310 rice blast isolates from the Rice Blast Nursery at the International Rice Research Institute was amplified using a pair of primers designed on the basis of an inverted repeat transposon Pot?2 sequence of Magnaporthe grisea . The isolates were divided into 6 lineages according to the DNA band data at the level of 80% similarity. The lineages were closely related to genetic background of rice cultivars and varied with rice growth period.\;