表皮形态发生素对肝细胞癌SK-HEP-1细胞生物学活性的影响

Effects of Epimorphin on SK-HEP-1 Cell Biology

目的:明确表皮形态发生素(EPM)对肝细胞癌SK-HEP-1细胞生物学行为的影响。方法:构建高表达EPM的SK-HEP-1细胞,real-time PCR和Western印迹检测EPM在肿瘤细胞内的表达,CCK8分析和克隆形成实验检测细胞的增殖能力,Matrigel-transwell实验检测细胞的浸润能力。结果:EPM在肿瘤细胞内的高表达不影响细胞的增殖能力,但明显增强肿瘤细胞的浸润能力。结论:肝癌肿瘤微环境有可能通过EPM影响肿瘤细胞的生物学活性,对其作用机制的进一步明确,将有助于阐明肝癌发生发展的病理机制,发现新的恶性肿瘤诊断和治疗手段。

Objective： To study whether epimorphin（EPM） affect SK-HEP-1 cell biology. Methods： SK-HEP-1 ceils with ectopic EPM expression were constructed and identified by real-time PCR and Western blot. CCK8 analysis and colony formation assay were used to detect cancer cell proliferation. The invasion activity of cancer cells were detected by Matrigel-transwell assay. Results： We first confirmed with that EPM was unregulated in hepatocellular carcinoma（HCC） tumor stroma tissues. And, the further results suggested that ectopic expression of EPM in SK-HEP-1 cell had no effects on cancer cell proliferation, but enhanced the cell invasion activity significantly. We also showed that EPM could enhance FAK activation significantly. Conclusion： Taken together, our results re- veal that EPM was involved in HCC progression and may be a potential therapeutic target for HCC.