汉口、汉阳两地空气细颗粒物PM_(2.5)遗传毒性研究

Study of Genotoxicity of PM_(2.5) in Hanyang and Hanhou Regions

目的:探究汉口、汉阳两地空气细颗粒物PM2.5的遗传毒性。方法:采用Ames波动实验和双核细胞微核实验评价PM2.5在基因和染色体水平是否具有遗传毒性。结果:Ames波动实验结果表明,汉口和汉阳PM2.5对鼠伤寒沙门菌TA100无致突变作用;而在双核细胞微核实验中,两地PM2.5随浓度上升微核率逐渐上升,呈剂量反应关系,对染色体有损伤作用。2项实验结果都显示,汉口和汉阳两地的颗粒物在致遗传性上无明显差异。结论:汉口、汉阳两地PM2.5具有一定的潜在遗传毒性。

Objective： To study the genotoxicity of PM25 in Hankou and Hanyang regions. Methods： Ames fluctuation test and cell micronucleus test were used to evaluate the genotoeixity of PM2.5 Results： No mutation effects of PM2.5 were detected in Ames fluctuation test. However, the micronucleus rates induced by PM2.5 were increased in a dose dependent manner. The two results showed that there was no clear difference of genotoxicity between the two regions. Conclusion： The PM2.5 in Hankou and Hanyang has genotoxieity potentially.