吉林省延边地区羊泰勒虫病的分子流行病学调查

Molecular epidemiological survey of ovine and caprine theileriosis in Yanbian area

为了解羊泰勒虫病在吉林省延边地区的流行情况,本研究采用PCR方法和血液涂片染色镜检法对吉林省延边地区延吉市、龙井市、和龙市、珲春市4个地区采集的93份羊血液样品进行检测,并对不同地区、饲养方式、地理条件以及不同年龄间羊泰勒虫病的感染情况进行比较分析。结果显示,PCR方法检测样品的阳性率为31.18%,显著高于血涂片染色镜检法19.35%的阳性率。经统计学分析,不同地区和不同品种,羊泰勒虫感染率差异不显著(p>0.05);而不同年龄阶段和不同饲养方式,羊泰勒虫的感染率差异显著(p<0.05)。调查结果表明,吉林省延边地区是羊泰勒虫病的流行地区。

To investigate the prevalence of ovine and caprine theileriosis in Yanbian area, a molecular epidemiological survey was conducted to examine Theileria using PCR method. A total of 93 blood samples were collected from Yanji, Longjing, Helong, Hunchun in Yanbian area. Risk factors such as regions, grazing, geographical conditions and ages were evaluated and statistical ananlysis was carried out. The results showed that the positive rate by PCR method was 31.18% which was significantly higher than 19.35% by microscopic examination of blood smears. The results indicated that age and grazing were associated with the prevalence of ovine and caprine theileriosis （p〈0.05）, but not with regions and breeds （p〉0.05）. The survey confirmed that Yanbian area was the epidemic region of ovine and caprine theileriosis.